Western Blot (WB)
(WB Suggested Anti-H3f3a AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Spleen)
Rabbit H3f3a Polyclonal Antibody | anti-H3F3A antibody
H3f3a antibody - N-terminal region
Gene Names
H3f3a; H3.3A; EyeLinc14
Reactivity
Dog, Pig, Rat, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Synonyms
H3f3a; Polyclonal Antibody; H3f3a antibody - N-terminal region; anti-H3F3A antibody
Host
Rabbit
Reactivity
Dog, Pig, Rat, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: KSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQ
Sequence Length
126
Applicable Applications for anti-H3F3A antibody
Western Blot (WB)
Homology
Dog: 100%; Pig: 100%; Rat: 100%; Zebrafish: 100%
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(WB Suggested Anti-H3f3a AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Spleen)
Western Blot (WB)
(WB Suggested Anti-H3f3a AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Spleen)
Related Product Information for anti-H3F3A antibody
This is a rabbit polyclonal antibody against H3f3a. It was validated on Western Blot
Target Description: H3f3a is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. H3f3a constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. H3f3a is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Target Description: H3f3a is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. H3f3a constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. H3f3a is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Product Categories/Family for anti-H3F3A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15kDa
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone, family 3A
NCBI Official Symbol
H3f3a
NCBI Official Synonym Symbols
H3.3A; EyeLinc14
NCBI Protein Information
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3f3a
UniProt Synonym Gene Names
H3.3a
H3f3b; H3.3b
H3f3b; H3.3b
UniProt Entry Name
H33_MOUSE
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Nov 2015]
Research Articles on H3F3A
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Product Notes
The H3F3A h3f3a (Catalog #AAA3215040) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H3f3a antibody - N-terminal region reacts with Dog, Pig, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's H3f3a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the H3F3A h3f3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ. It is sometimes possible for the material contained within the vial of "H3f3a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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