Typical Standard Curve/Testing Data
Choline Assay Kit
Choline Assay Kit (Fluorometric)
Synonyms
Choline; Choline Assay Kit (Fluorometric); Choline assay kit
Preparation and Storage
Upon receipt, store the Microtiter Plate and Assay Buffer at 4 degree C. Store all other components at -20 degree C. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Choline assay kit
Principle of the Assay: Choline Assay Kit measures choline present within serum, plasma, and other tissue samples. The assay is based on an enzyme driven reaction that will detect choline via choline oxidase activity. Choline is oxidized by choline oxidase to produce hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorescence probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of choline standard within the 96-well microtiter plate format. Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Background/Introduction: Choline is a water soluble amine that is an essential nutrient. Choline and its metabolites are important to cell signaling, cholinergic neurotransmission, and cell membrane structural integrity. The metabolite is categorized as a B-complex vitamin that is synthesized by the human body, but not in sufficient quantity for proper health. It is a key precursor to the phospholipids phosphatidylcholine and sphingomyelin, which are abundant in cell membranes. During the perinatal period, choline is transferred to infants via breast milk to assist in brain and growth development. The neurotransmitter acetylcholine, which is intricate to the central and peripheral nervous systems, is produced from choline. Choline disorders can have a profound impact on neurological function. Choline deficiency has been implicated in atherosclerosis, liver disease, cancers, as well as neurological disorders. Neural tube and memory defects in infants have been linked with low choline levels in pregnant women. More research of choline is needed to elucidate its complex roles within the body and its relation to disease states. Choline Assay Kit is a simple fluorometric assay that measures the amount of choline present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards and samples. Sample choline concentrations are determined by comparison with a known choline standard. The kit's detection sensitivity limit is approximately 75 nM choline.
Background/Introduction: Choline is a water soluble amine that is an essential nutrient. Choline and its metabolites are important to cell signaling, cholinergic neurotransmission, and cell membrane structural integrity. The metabolite is categorized as a B-complex vitamin that is synthesized by the human body, but not in sufficient quantity for proper health. It is a key precursor to the phospholipids phosphatidylcholine and sphingomyelin, which are abundant in cell membranes. During the perinatal period, choline is transferred to infants via breast milk to assist in brain and growth development. The neurotransmitter acetylcholine, which is intricate to the central and peripheral nervous systems, is produced from choline. Choline disorders can have a profound impact on neurological function. Choline deficiency has been implicated in atherosclerosis, liver disease, cancers, as well as neurological disorders. Neural tube and memory defects in infants have been linked with low choline levels in pregnant women. More research of choline is needed to elucidate its complex roles within the body and its relation to disease states. Choline Assay Kit is a simple fluorometric assay that measures the amount of choline present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards and samples. Sample choline concentrations are determined by comparison with a known choline standard. The kit's detection sensitivity limit is approximately 75 nM choline.
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Product Notes
The Choline (Catalog #AAA169291) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Choline, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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