Serum Triglyceride Assay Kit

Serum Triglyceride Quantification Kit (Fluorometric)

Cat. Num. AAA168773

• Synonyms: Serum Triglyceride; Serum Triglyceride Quantification Kit (Fluorometric); Serum Triglyceride assay kit

• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store entire kit at -80 degree C. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Serum Triglyceride assay kit

Background: Triglycerides (TAG) are a type of lipid in the blood, serving as an energy source and playing a key role in metabolism. Triglycerides are the digestive end product of breaking down dietary fats. Any extra carbohydrates and fats that are not immediately used are chemically converted into triglycerides. In the intestines, secreted enzyme lipases hydrolyse the triglyceride ester bond, yielding glycerol and free fatty acids in a process called lipolysis. Enterocytes then absorb and repackage the fragments with cholesterol to form chylomicrons, a major lipoprotein transport particle. In the liver, hepatic lipases also break down triglycerides to assemble another lipoprotein particle (VLDL) from triglycerides, cholesterol, and apolipoproteins. Serum Triglyceride Quantification Kit measures triglyceride concentration in serum, plasma, and lysates by a coupled enzymatic reaction system. First, lipase hydrolyzes the triglyceride ester bond, yielding glycerol. The glycerol is then phosphorylated and oxidized, producing hydrogen peroxide which reacts with the kit's Fluorometric Probe (Ex. 530-560 nm/Em. 585-595 nm). The Serum Triglyceride Quantification Kit is a simple, fluorometric assay that quantitatively measures the amount of triglyceride in plasma, serum, and lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, triglyceride standards, free glycerol controls and unknown samples. The kit contains a triglyceride standard and has a detection sensitivity limit of ~2 muM (0.2 mg/dL).

Product Categories/Family for Serum Triglyceride assay kit

Metabolism Research; Lipoprotein Metabolism; Triglyceride Assays

References

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