Typical Testing Data/Standard Curve (for reference only)
Glucose Assay Kit
Glucose Assay Kit (Fluorometric)
Synonyms
Glucose; Glucose Assay Kit (Fluorometric); Glucose assay kit
Preparation and Storage
Upon receipt, store the Glucose Standard, Colorimetric Probe, HRP, and Glucose Oxidase at -20 degree C
The Fluorescence Probe is light sensitive and must be stored accordingly
Avoid multiple freeze/thaw cycles
Store the 10X Assay Buffer at room temperature.
The Fluorescence Probe is light sensitive and must be stored accordingly
Avoid multiple freeze/thaw cycles
Store the 10X Assay Buffer at room temperature.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Glucose assay kit
Principle of the Assay: Total Glucose Assay Kit measures total glucose within food or biological samples. Glucose is oxidized by glucose oxidase into D-gluconic acid plus hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of glucose standard within the 96-well microtiter plate format. Samples and standards are incubated for 45 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Background: Glucose is a sugar used as an important source of energy in plants, prokaryotes and eukaryotes via processes such as respiration and fermentation. In plants, algae, and cyanobacteria, the energy of light is synthesized into the storage form of sugars such as glucose. More specifically, in a downstream process known as the Calvin cycle, carbon dioxide is incorporated into organic carbon compounds, like ribulose bisphosphate. Using ATP and NADPH from upstream light-dependent reactions, the resulting compounds are then reduced and removed to form further carbohydrates, such as glucose. In animals, through the process of glycolysis followed by the citric acid cycle, glucose is broken down to water and CO2, resulting in energy from ATP formation. Glucose is often stored as a polymer such as glycogen. In humans, glucose is commonly measured in blood samples. Bloodstream levels of glucose are normally under tight regulation (Table 1); however, high levels measured in fasting individuals may indicate prediabetes or diabetes. Glucose Assay Kit is a simple fluorometric assay that measures the amount of total glucose present in foods or biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 500 assays, including blanks, glucose standards and unknown samples. Sample glucose concentrations are determined by comparison with a known glucose standard. The kit has a detection sensitivity limit of 1.56 uM glucose.
Background: Glucose is a sugar used as an important source of energy in plants, prokaryotes and eukaryotes via processes such as respiration and fermentation. In plants, algae, and cyanobacteria, the energy of light is synthesized into the storage form of sugars such as glucose. More specifically, in a downstream process known as the Calvin cycle, carbon dioxide is incorporated into organic carbon compounds, like ribulose bisphosphate. Using ATP and NADPH from upstream light-dependent reactions, the resulting compounds are then reduced and removed to form further carbohydrates, such as glucose. In animals, through the process of glycolysis followed by the citric acid cycle, glucose is broken down to water and CO2, resulting in energy from ATP formation. Glucose is often stored as a polymer such as glycogen. In humans, glucose is commonly measured in blood samples. Bloodstream levels of glucose are normally under tight regulation (Table 1); however, high levels measured in fasting individuals may indicate prediabetes or diabetes. Glucose Assay Kit is a simple fluorometric assay that measures the amount of total glucose present in foods or biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 500 assays, including blanks, glucose standards and unknown samples. Sample glucose concentrations are determined by comparison with a known glucose standard. The kit has a detection sensitivity limit of 1.56 uM glucose.
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Product Notes
The Glucose (Catalog #AAA169260) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glucose, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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