Typical Testing Data/Standard Curve (for reference only)
D-Amino Acid Assay Kit
D-Amino Acid Assay Kit (Fluorometric)
Synonyms
D-Amino Acid; D-Amino Acid Assay Kit (Fluorometric); D-Amino Acid assay kit
Preparation and Storage
Upon receipt, store the D-Amino Acid Oxidase at -80 C. Store all other components at -20 C. The Colorimetric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for D-Amino Acid assay kit
Principle of the Assay: Cell Biolabs' D-Amino Acid Assay Kit (Fluorometric) measures free D-Amino Acids (except for D-Glutamate and D-Aspartate) present in foods or biological samples in a 96-well microtiter plate format. D-amino acids are oxidatively deaminated by D-Amino Acid oxidase into the corresponding alpha-keto acid, ammonia, and hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples and standards are read with a standard 96-well fluorometric plate reader. Samples are compared to a known concentration of D-Alanine standard within the 96-well microtiter plate format (Figure 1).
Background/Introduction: D-Amino acids are amino acids where the stereogenic carbon, or chiral carbon, next to the amino group has the D-configuration. "D" stands for dextrorotary, meaning that the isomer rotates plane polarized light clockwise. For most naturally-occurring amino acids, this carbon has the L-configuration, although D-Amino acids are sometimes found as residues in proteins. D-Amino acids have been found in various foods such as fruits, vegetables, milk, cheese, meats, and fish. While D-amino acids are not detected in human serum, micromolar levels can be detected in the mammalian brain and endocrine systems of humans and mice. For example, D-serine is released from neurons, through alanine-serine-cysteine transporter-1 (Asc-1) or other pathways, into the synapse where it can bind and affect NMDA receptor activity. D-serine induces long term potentiation (LTP, a model of learning and memory), and breakdown of D-serine by D-amino acid oxidase causes suppression of LTP induction. Aberrant levels of D-serine are associated with diseases caused by abnormal NMDA receptor activity. Increased levels of D-serine in the cerebrospinal fluid (CSF) have been detected in patients with Alzheimer's disease compared to normal controls. Decreased D-serine levels (which lowers NMDA receptor signaling) is associated with schizophrenia-like symptoms. Cell Biolabs' D-Amino Acid Assay Kit (Fluorometric) is a simple fluorometric assay that measures the total amount of free D-Amino Acids present in biological samples (except for D-glutamate and D-aspartate) in a 96-well microtiter plate format. Amino Acids found in polypeptide chains (peptides and proteins) are not detected. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, standards and unknown samples. Sample D-Amino Acid concentrations are determined by comparison with a known D-Alanine standard. The kit has a detection sensitivity limit of 1.56 uM D-Amino Acids.
Background/Introduction: D-Amino acids are amino acids where the stereogenic carbon, or chiral carbon, next to the amino group has the D-configuration. "D" stands for dextrorotary, meaning that the isomer rotates plane polarized light clockwise. For most naturally-occurring amino acids, this carbon has the L-configuration, although D-Amino acids are sometimes found as residues in proteins. D-Amino acids have been found in various foods such as fruits, vegetables, milk, cheese, meats, and fish. While D-amino acids are not detected in human serum, micromolar levels can be detected in the mammalian brain and endocrine systems of humans and mice. For example, D-serine is released from neurons, through alanine-serine-cysteine transporter-1 (Asc-1) or other pathways, into the synapse where it can bind and affect NMDA receptor activity. D-serine induces long term potentiation (LTP, a model of learning and memory), and breakdown of D-serine by D-amino acid oxidase causes suppression of LTP induction. Aberrant levels of D-serine are associated with diseases caused by abnormal NMDA receptor activity. Increased levels of D-serine in the cerebrospinal fluid (CSF) have been detected in patients with Alzheimer's disease compared to normal controls. Decreased D-serine levels (which lowers NMDA receptor signaling) is associated with schizophrenia-like symptoms. Cell Biolabs' D-Amino Acid Assay Kit (Fluorometric) is a simple fluorometric assay that measures the total amount of free D-Amino Acids present in biological samples (except for D-glutamate and D-aspartate) in a 96-well microtiter plate format. Amino Acids found in polypeptide chains (peptides and proteins) are not detected. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, standards and unknown samples. Sample D-Amino Acid concentrations are determined by comparison with a known D-Alanine standard. The kit has a detection sensitivity limit of 1.56 uM D-Amino Acids.
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Product Notes
The D-Amino Acid (Catalog #AAA169595) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "D-Amino Acid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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