Typical Testing Data/Standard Curve (for reference only)
General Malate (Fluorometric) Assay Kit
Malate Assay Kit (Fluorometric)
Reactivity
General
Synonyms
Malate (Fluorometric); Malate Assay Kit (Fluorometric); Malate (Fluorometric) assay kit
Reactivity
General
Preparation and Storage
Upon receipt, store the 10X Assay Buffer and the L-Malic Acid Dehydrogenase at 4 degree C (DO NOT FREEZE L-Malic Dehydrogenase). Store the L-Malate Standard and the Assay Reagent at -80 degree C and avoid multiple freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Malate (Fluorometric) assay kit
Principle of the Assay: The Malate Acid Assay Kit (Fluorometric) measures the malate within beverages, food, serum, plasma, and cell or tissue lysate samples. The assay is based on an enzyme driven reaction: when malate incubated in the presence of Malic Acid dehydrogenase (MDH) and NAD+, NAD+ is converted to its reduced form NADH. Diaphorase then uses NADH to reduce resazurin to resorufin which is then detected fluorometrically (Figure 1).
Background/Introduction: Malate, the anion salt of malic acid, is a dicarboxylic acid that is found in all living organisms. Malate plays an important role in biochemistry. In the process of C4 carbon fixation, malate provides CO2 in the Calvin cycle. In the Krebs cycle, malate is created by the addition of an -OH group to fumarate. Malate can additionally be formed from pyruvate by anaplerotic reactions. Malate is also created in the guard cells of plant leaves by phosphoenolpyruvate carboxylation. Malate is often added to various foods and therefore quantitation of malate is important in various industries such as fruit, wine, beer, and cheese manufacturing. In the fruit industry, levels of malic acid have been found to be higher in organic crops as opposed to other standard methods of agriculture.
Background/Introduction: Malate, the anion salt of malic acid, is a dicarboxylic acid that is found in all living organisms. Malate plays an important role in biochemistry. In the process of C4 carbon fixation, malate provides CO2 in the Calvin cycle. In the Krebs cycle, malate is created by the addition of an -OH group to fumarate. Malate can additionally be formed from pyruvate by anaplerotic reactions. Malate is also created in the guard cells of plant leaves by phosphoenolpyruvate carboxylation. Malate is often added to various foods and therefore quantitation of malate is important in various industries such as fruit, wine, beer, and cheese manufacturing. In the fruit industry, levels of malic acid have been found to be higher in organic crops as opposed to other standard methods of agriculture.
Product Categories/Family for Malate (Fluorometric) assay kit
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Product Notes
The General Malate (Fluorometric) (Catalog #AAA169576) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA169576 Assay Kit recognizes General Malate (Fluorometric). It is sometimes possible for the material contained within the vial of "Malate (Fluorometric), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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