Rabbit FER Polyclonal Antibody | anti-FER antibody

FER Antibody

Cat. Num. AAA9611025

• Gene Names: FER; TYK3; PPP1R74; p94-Fer
• Reactivity: Human, Mouse; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FER; Polyclonal Antibody; FER Antibody; c FER; Feline encephalitis virus related kinase FER; Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94); Fer (fps/fes related) tyrosine kinase; FER_HUMAN; FerT; Fujinami poultry sarcoma/Feline sarcoma related protein Fer; p94 FER; p94-FER; Pe1Fe10; Pe1Fe13; Pe1Fe3; Pe1Fe6; Phosphoprotein NCP94; PPP1R74; Protein phosphatase 1 regulatory subunit 74; Proto oncogene tyrosine protein kinase FER; Proto-oncogene c-Fer; TYK3; Tyrosine kinase 3; Tyrosine-protein kinase Fer; anti-FER antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FER Antibody detects endogenous levels of FER.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 822

• Applicable Applications for anti-FER antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human FER.
• Tissue Specificity: Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.
• Subcellular Location: Cytoskeleton; Plasma Membrane; Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of FER in lysates of Jurkat , using FER Antibody.)

Related Product Information for anti-FER antibody

Description: Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus.

Post Translational Modifications: Autophosphorylated. Polyubiquitinated; this leads to proteasomal degradation.

Subunit Structure: Homotrimer. Interacts with ARHGDIA, IRS1, JAK1, NRP1, PIK3R1, PLEC and TMF1. Interacts with PPP1CA and regulates its phosphorylation at 'Thr-320' (By similarity). Interacts with CTNND1, EGFR, FLT3, PECAM1, PDGFR and STAT3. Interacts (via SH2 domain) with CTTN. Interacts with HSP90; this stabilizes phosphorylated FER and protects FER against proteasomal degradation. Component of a complex that contains at least FER, CTTN and PTK2/FAK1.

Similarity: The coiled coil domains mediate homooligomerization and are required for location at microtubules. The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes. Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 119964721
• NCBI GeneID: 2241
• NCBI Accession #: NP_005237.2
• NCBI GenBank Nucleotide #: NP_005237.2
• UniProt Accession #: P16591
• Molecular Weight: Observed: 95 kDa; Predicted: 95 kDa
• NCBI Official Full Name: tyrosine-protein kinase Fer isoform a
• NCBI Official Synonym Full Names: FER tyrosine kinase
• NCBI Official Symbol: FER
• NCBI Official Synonym Symbols: TYK3; PPP1R74; p94-Fer
• NCBI Protein Information: tyrosine-protein kinase Fer
• UniProt Protein Name: Tyrosine-protein kinase Fer
• Protein Family: Ferritin
• UniProt Gene Name: FER
• UniProt Synonym Gene Names: TYK3
• UniProt Entry Name: FER_HUMAN

NCBI Description

The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]

Uniprot Description

Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.

Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Oncoprotein; EC 2.7.10.2; TK group; Fer family

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: microtubule cytoskeleton; extrinsic to internal side of plasma membrane; lamellipodium; cytoplasm; nuclear chromatin; cell cortex; cytosol; nucleus; cell junction; actin cytoskeleton

Molecular Function: protein binding; cadherin binding; gamma-catenin binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; actin binding; lipid binding; protein kinase binding; protein phosphatase 1 binding; Rab GTPase binding; ATP binding; epidermal growth factor receptor binding

Biological Process: regulation of mast cell degranulation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; diapedesis; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; negative regulation of mast cell activation during immune response; chemotaxis; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of cell proliferation; cell adhesion; cell differentiation; regulation of protein amino acid phosphorylation; cytokine and chemokine mediated signaling pathway; microtubule cytoskeleton organization and biogenesis; regulation of epidermal growth factor receptor signaling pathway; regulation of cell proliferation; cell proliferation; positive regulation of actin filament polymerization; cellular response to insulin stimulus; actin cytoskeleton reorganization; innate immune response; mitotic cell cycle; positive regulation of cell migration