Typical Testing Data/Standard Curve (for reference only)
Lipase Assay Kit | PNLIP assay kit
Lipase Activity Colorimetric Assay Kit
Fast and convenient
Sensitive assays for measuring Lipase activity in various biological samples.
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help identify Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours. In MyBioSource's Lipase Assay Kit, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (lambda=570nm). This assay is rapid, simple, sensitive, and reliable, as well as, suitable for high throughput activity screening of lipase. This kit detects lipase activity as low as 10mU per well.
Summary: Detection method-Absorbance (570 nm)
Species reactivity- Mammalian
Applications- This kit detects lipase activity as low as 10mU per well.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]
Uniprot Description
PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]
Protein type: Hydrolase; Lipid Metabolism - glycerolipid; EC 3.1.1.3; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q26.1
Cellular Component: extracellular region
Molecular Function: triacylglycerol lipase activity; metal ion binding
Biological Process: phototransduction, visible light; cholesterol absorption; retinoid metabolic process; lipid catabolic process; lipid digestion
Disease: Pancreatic Lipase Deficiency
Research Articles on PNLIP
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Product Notes
The PNLIP pnlip (Catalog #AAA841708) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Lipase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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