Typical Testing Data/Standard Curve (for reference only)
Nampt (Visfatin/PBEF) ELISA Kit
Nampt (Visfatin / PBEF) (mouse / rat) Serum ELISA Kit
Synonyms
Nampt (Visfatin/PBEF); Nampt (Visfatin / PBEF) (mouse / rat) Serum ELISA Kit; Nampt (Visfatin/PBEF) elisa kit
Samples
Serum (mouse/ rat)
Assay Type
Sandwich
Features & Benefits
Simple procedure
Fast and convenient
High-throughput adaptable
Fast and convenient
High-throughput adaptable
Preparation and Storage
4 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Nampt (Visfatin/PBEF) elisa kit
Product Information: Nampt (Visfatin/PBEF) (mouse/rat)Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of mouse or rat Nampt (Visfatin/PBEF) in serum. Assay range ~0.5 - 32 ng Nampt/ml and detection limit 50 pg/ml. 100 Assays.
Background: Visfatin, an adipocytokine that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin, a secretory form of Nampt (nicotinamide phosphoribosy-ltransferase), corresponds to pre-B cell colony-enhancing factor (PBEF), a 52 kDa cytokine expressed in lymphocytes. PBEF is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. The Nampt (Visfatin/PBEF) (mouse/rat) Serum ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat Nampt (Visfatin/PBEF) in serum. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat Nampt. It does not cross-react with human Nampt, human adiponectin, mouse adiponectin, mouse resistin, mouse vaspin, mouse RBP4, mouse GPX3, mouse progranulin, mouse IL-33, mouse clusterin, mouse ANGPTL3, mouse ANGPTL4, mouse ANGPTL6, mouse leptin, mouse TNF-alpha. The assay range is 0.5 - 32 ng Nampt/ml and a detection limit of 50 pg/ml (based on adding two standard deviations to the mean value of the zero standard).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications-The assay detects Visfatin from serum samples in the range from 0.5 - 32 ng Visfatin/ml and a detection limit of 50 pg/ml.
Background: Visfatin, an adipocytokine that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin, a secretory form of Nampt (nicotinamide phosphoribosy-ltransferase), corresponds to pre-B cell colony-enhancing factor (PBEF), a 52 kDa cytokine expressed in lymphocytes. PBEF is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. The Nampt (Visfatin/PBEF) (mouse/rat) Serum ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat Nampt (Visfatin/PBEF) in serum. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat Nampt. It does not cross-react with human Nampt, human adiponectin, mouse adiponectin, mouse resistin, mouse vaspin, mouse RBP4, mouse GPX3, mouse progranulin, mouse IL-33, mouse clusterin, mouse ANGPTL3, mouse ANGPTL4, mouse ANGPTL6, mouse leptin, mouse TNF-alpha. The assay range is 0.5 - 32 ng Nampt/ml and a detection limit of 50 pg/ml (based on adding two standard deviations to the mean value of the zero standard).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications-The assay detects Visfatin from serum samples in the range from 0.5 - 32 ng Visfatin/ml and a detection limit of 50 pg/ml.
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Product Notes
The Nampt (Visfatin/PBEF) (Catalog #AAA843345) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nampt (Visfatin/PBEF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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