Typical Testing Data/Standard Curve (for reference only)
Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit
Nampt (Visfatin / PBEF) (human) Intracellular ELISA Kit
Synonyms
Nampt (Visfatin/PBEF) (human) Intracellular; Nampt (Visfatin / PBEF) (human) Intracellular ELISA Kit; Nampt (Visfatin/PBEF) (human) Intracellular elisa kit
Samples
Cell lysates
Assay Type
Sandwich
Features & Benefits
Simple procedure
Fast and convenient
High-throughput adaptable
Fast and convenient
High-throughput adaptable
Preparation and Storage
4 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Nampt (Visfatin/PBEF) (human) Intracellular elisa kit
Product Information: Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of intracellular human Nampt (Visfatin/PBEF). Assay range 0.063 - 16 ng Nampt/ml and detection limit 30 pg/ml. 100 Assays.
Background: Nampt, nicotinamide phosphoribosy-ltransferase, is the rate limiting enzyme of the mammalian NAD biosynthesis pathway. The circulating NMN (nicotinamide mononucleotide) and NAD are taken up by beta cells and converted to NAD by Nmnat (nicotinamide mononucleotide adenyltransferase) and intracellular Nampt. The Nampt plays a critical role in enhancing life span and protecting against oxidative damage. The Nampt (Visfatin/PBEF) (human) Intracelluar ELISA Kit is to be used for the in vitro quantitative determination of intracellular human Nampt (Visfatin/PBEF). This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples. This ELISA is specific for the measurement of natural and recombinant human Nampt. It does not cross-react with human adiponectin, human resistin, human RELM-beta, human leptin, human GPX3, human ANGPTL4, human FABP4, human ANGPTL6, human PAI1. The assay range is 0.063 - 16 ng Nampt/ml and a detection limit of 30 pg/ml (based on adding two standard deviations to the mean value of the zero standard).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications-The assay detects Visfatin from serum samples in the range from 0.063 - 16 ng Visfatin/ml and a detection limit of 30 pg/ml.
Background: Nampt, nicotinamide phosphoribosy-ltransferase, is the rate limiting enzyme of the mammalian NAD biosynthesis pathway. The circulating NMN (nicotinamide mononucleotide) and NAD are taken up by beta cells and converted to NAD by Nmnat (nicotinamide mononucleotide adenyltransferase) and intracellular Nampt. The Nampt plays a critical role in enhancing life span and protecting against oxidative damage. The Nampt (Visfatin/PBEF) (human) Intracelluar ELISA Kit is to be used for the in vitro quantitative determination of intracellular human Nampt (Visfatin/PBEF). This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples. This ELISA is specific for the measurement of natural and recombinant human Nampt. It does not cross-react with human adiponectin, human resistin, human RELM-beta, human leptin, human GPX3, human ANGPTL4, human FABP4, human ANGPTL6, human PAI1. The assay range is 0.063 - 16 ng Nampt/ml and a detection limit of 30 pg/ml (based on adding two standard deviations to the mean value of the zero standard).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications-The assay detects Visfatin from serum samples in the range from 0.063 - 16 ng Visfatin/ml and a detection limit of 30 pg/ml.
Product Categories/Family for Nampt (Visfatin/PBEF) (human) Intracellular elisa kit
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Product Notes
The Nampt (Visfatin/PBEF) (human) Intracellular (Catalog #AAA843329) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nampt (Visfatin/PBEF) (human) Intracellular, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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