Apolipoprotein H ELISA Pair Set | APOH elisa pair set

Apolipoprotein H Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118362

• Synonyms: Apolipoprotein H; Apolipoprotein H Matched ELISA Antibody Pair Set; Human; Human Apolipoprotein H/APOH ELISA Pair Set; apolipoprotein H (beta-2-glycoprotein I); B2G1 Matched ELISA Antibody Pair Set; B2GP1 Matched ELISA Antibody Pair Set; BG Matched ELISA Antibody Pair Set; APOH elisa pair set

• Specificity: Quantitative determination of Human Apolipoprotein H

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 39.06-2500pg/mL
• Sensitivity: 39.06pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-Human Apolipoprotein H/APOH monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 0.5ug/mL in PBS before coating.2. Detection Antibody 0.2 mg/mL of mouse anti-Human Apolipoprotein H/APOH monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.1ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 20 ng of recombinant Human Apolipoprotein H/APOH. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for APOH elisa pair set

Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. APOH appears to completely inhibit serotonin release by the platelets and prevents subsequent waves of the ADP-induced aggregation. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH).

This Apolipoprotein H Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Apolipoprotein H. It contains Human Apolipoprotein H capture antibody, Human Apolipoprotein H detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Apolipoprotein H protein.

References

Kamboh MI, et al. (1998) Genetics of apolipoprotein H (beta2-glycoprotein I) and anionic phospholipid binding. Lupus. 7 Suppl 2: S10-3.Singh P, et al. (2002) Genetics of apolipoprotein H (beta2-glycoprotein I) polymorphism in India. Ann Hum Biol. 29(3): 247-55.Xia J, et al. (2004) Apolipoprotein H gene polymorphisms and risk of primary cerebral hemorrhage in a Chinese population. Cerebrovasc Dis. 17(2-3): 197-203.Chen Q, et al. (2006) Complete DNA sequence variation in the apolipoprotein H (beta-glycoprotein I) gene and identification of informative SNPs. Ann Hum Genet. 70(Pt 1): 1-11.Leduc MS, et al. (2008) Comprehensive evaluation of apolipoprotein H gene (APOH) variation identifies novel associations with measures of lipid metabolism in GENOA. J Lipid Res. 49(12): 2648-56.