Apolipoprotein H Active Protein | APOH active protein

Apolipoprotein H Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120745

• Gene Names: APOH; BG; B2G1; B2GP1
• Purity: >96% as determined by SDS-PAGE
• Synonyms: Apolipoprotein H; Apolipoprotein H Protein; Human; Recombinant (His Tag); Human Apolipoprotein H/APOH Protein (His Tag); B2G1 Protein; B2GP1 Protein; BG Protein; apolipoprotein H (beta-2-glycoprotein I); APOH active protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys345

• Species: Human
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human APOH-his at 2ug/mL (100uL/well) can bind biotinylated human LDLR-his. The EC50 of biotinylated human LDLR-his is 0.35ug/mL.; 3.Immobilized human APOH-his at 2ug/mL (100uL/well) can bind biotinylated mouse LDLR-his. The EC50 of biotinylated mouse LDLR-his is 26ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human APOH (NP_000033.2)(Met 1-Cys 345) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human APOH-his at 2 ug/mL (100 ul/well) can bind biotinylated human LDLR-his, The EC50 of biotinylated human LDLR-his is 0.35 ug/mL.)

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human APOH-his at 2 ug/mL (100 ul/well) can bind biotinylated mouse LDLR-his, The EC50 of biotinylated mouse LDLR-his is 26 ng/mL.)

Related Product Information for APOH active protein

Background: Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. APOH appears to completely inhibit serotonin release by the platelets and prevents subsequent waves of the ADP-induced aggregation. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH).

References

Kamboh MI, et al. (1998) Genetics of apolipoprotein H (beta2-glycoprotein I) and anionic phospholipid binding. Lupus. 7 Suppl 2: S10-3.Singh P, et al. (2002) Genetics of apolipoprotein H (beta2-glycoprotein I) polymorphism in India. Ann Hum Biol. 29(3): 247-55.Xia J, et al. (2004) Apolipoprotein H gene polymorphisms and risk of primary cerebral hemorrhage in a Chinese population. Cerebrovasc Dis. 17(2-3): 197-203.Chen Q, et al. (2006) Complete DNA sequence variation in the apolipoprotein H (beta-glycoprotein I) gene and identification of informative SNPs. Ann Hum Genet. 70(Pt 1): 1-11.Leduc MS, et al. (2008) Comprehensive evaluation of apolipoprotein H gene (APOH) variation identifies novel associations with measures of lipid metabolism in GENOA. J Lipid Res. 49(12): 2648-56.

NCBI and Uniprot Product Information

• NCBI GI #: 153266841
• NCBI GeneID: 350
• NCBI Accession #: NP_000033.2
• NCBI GenBank Nucleotide #: NM_000042.2
• UniProt Accession #: P02749; Q9UCN7; B2R9M3
• Molecular Weight: 48000
• NCBI Official Full Name: beta-2-glycoprotein 1
• NCBI Official Synonym Full Names: apolipoprotein H (beta-2-glycoprotein I)
• NCBI Official Symbol: APOH
• NCBI Official Synonym Symbols: BG; B2G1; B2GP1
• NCBI Protein Information: beta-2-glycoprotein 1; B2GPI; apo-H; beta(2)GPI; APC inhibitor; anticardiolipin cofactor; activated protein C-binding protein
• UniProt Protein Name: Beta-2-glycoprotein 1
• Protein Family: Beta-2-glycoprotein
• UniProt Gene Name: APOH
• UniProt Synonym Gene Names: B2G1; Apo-H; B2GPI; Beta(2)GPI
• UniProt Entry Name: APOH_HUMAN

NCBI Description

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq, Jul 2008]

Uniprot Description

APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface

Molecular Function: heparin binding; identical protein binding; protein binding; phospholipid binding; glycoprotein binding; lipid binding

Biological Process: negative regulation of angiogenesis; negative regulation of myeloid cell apoptosis; triacylglycerol metabolic process; positive regulation of blood coagulation; negative regulation of fibrinolysis; negative regulation of endothelial cell proliferation; positive regulation of lipoprotein lipase activity; negative regulation of blood coagulation; triacylglycerol transport; blood coagulation, intrinsic pathway; plasminogen activation; regulation of fibrinolysis