Pentraxin 3 ELISA Pair Set | PTX3 elisa pair set

Pentraxin 3 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118573

• Synonyms: Pentraxin 3; Pentraxin 3 Matched ELISA Antibody Pair Set; Human; Human Pentraxin 3/PTX3 ELISA Pair Set; pentraxin 3; PTX3 Matched ELISA Antibody Pair Set; TNFAIP5 Matched ELISA Antibody Pair Set; TSG-14 Matched ELISA Antibody Pair Set; PTX3 elisa pair set

• Specificity: Quantitative determination of Human Pentraxin 3

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 39.06-2500pg/mL
• Sensitivity: 39.06pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human Pentraxin 3/PTX3 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Pentraxin 3/PTX3 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 78 ng of recombinant Human Pentraxin 3/PTX3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for PTX3 elisa pair set

Pentraxin-related protein PTX3, also known as Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TSG-14, PTX3 and TNFAIP5, is a secreted protein that contains one pentaxin domain. PTX3 plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Pentraxins are a family of evolutionarily conserved multifunctional pattern-recognition proteins characterized by a cyclic multimeric structure. Based on the primary structure of the subunit, the pentraxins are divided into two groups: short pentraxins and long pentraxins. C-reactive protein (CRP) and serum amyloid P-component (SAP) are the two short pentraxins. The prototype protein of the long pentraxin group is pentraxin 3 (PTX3). CRP and SAP are produced primarily in the liver in response to IL-6, while PTX3 is produced by a variety of tissues and cells and in particular by innate immunity cells in response to proinflammatory signals and Toll-like receptor (TLR) engagement. PTX3 is essential in female fertility by acting as a nodal point for the assembly of the cumulus oophorus hyaluronan-rich extracellular matrix. PTX3 interacts with several ligands, including growth factors, extracellular matrix components and selected pathogens, playing a role in complement activation and facilitating pathogen recognition by phagocytes, acting as a predecessor of antibodies. PTX3 may also contribute to the pathogenesis of atherosclerosis.

This Pentraxin 3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Pentraxin 3. It contains Human Pentraxin 3 capture antibody, Human Pentraxin 3 detector antibody (HRP) and a highly purified recombinant Human Pentraxin 3 protein.

References

Rolph, M.S. et al., 2002, Arterioscler Thromb Vasc Biol. 22 (5):e10-4. Mantovani A., et al., 2003, Vaccine. 21:S43-S47. Luchetti, M.M. et al., 2004, Clin Exp Rheumatol. 22 (3):S66-72. Mantovani, A. et al., 2006, Vascul Pharmacol. 45 (5):326-30. Inforzato A., et al., 2008, J. Biol. Chem. 283:10147-61.