LIFR elisa pair set

LIFR Matched ELISA Antibody Pair Set, Rat

Cat. Num. AAA8118542

• Synonyms: LIFR; LIFR Matched ELISA Antibody Pair Set; Rat; Rat LIFR/CD118 ELISA Pair Set; leukemia inhibitory factor receptor alpha; LIFR elisa pair set

• Specificity: Quantitative determination of Rat LIFR

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 39.06-2500pg/mL
• Sensitivity: 39.06pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-Rat LIFR/CD118 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Rat LIFR/CD118 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 270 ng of recombinant Rat LIFR/CD118. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for LIFR elisa pair set

LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

This LIFR Matched ELISA Antibody Pair Set, Rat is a solid phase sandwich ELISA for quantitative determination of Rat LIFR. It contains Rat LIFR capture antibody, Rat LIFR detector antibody (HRP) and a highly purified HEK293-expressed recombinant Rat LIFR protein.

References

Gearing, D.P. et al., 1991, EMBO J. 10 (10): 2839-2848. Gearing, D.P. et al., 1992, New Biol. 4 (1): 61-65. Mosley, B. et al., 1996, J. Biol. Chem. 271 (51): 32635-32643. Timmermann, A. et al., 2002, Eur. J. Biochem. 269 (11): 2716-2726. Lass, A. et al., 2002, Fertil. Steril. 76 (6): 1091-1096.