RELT/TNFRSF19L ELISA Pair Set | RELT elisa pair set

RELT/TNFRSF19L Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118273

• Synonyms: RELT/TNFRSF19L; RELT/TNFRSF19L Matched ELISA Antibody Pair Set; Human; Human RELT/TNFRSF19L ELISA Pair Set; RELT tumor necrosis factor receptor; TNFRSF19L Matched ELISA Antibody Pair Set; TRLT Matched ELISA Antibody Pair Set; RELT elisa pair set

• Specificity: Quantitative determination of Human RELT/TNFRSF19L

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 39.06-2500pg/mL
• Sensitivity: 39.06pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human RELT/TNFRSF19L monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human RELT/TNFRSF19L monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 175 ng of recombinant Human RELT/TNFRSF19L. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for RELT elisa pair set

Receptor expressed in lymphoid tissues (RELT), also known as tumor necrosis factor receptor superfamily, member 19-like (TNFRSF19L), is a member of the TNF-receptor superfamily. This receptor is especially abundant in hematologic tissues. It has been shown to activate the NF-kappaB pathway and selectively bind TNF receptor-associated factor 1. RELT/TNFRSF19L is capable of stimulating T-cell proliferation in the presence of CD3 signaling, which suggests its regulatory role in immune response. RELT/TNFRSF19L is a type I transmembrane glycoprotein with a cysteine-rich extracellular domain, possessing significant homology to other members of the TNFR superfamily, especially TNFRSF19, DR3, OX40, and LTbeta receptor. RELT/TNFRSF19L is able to activate the NF-kappaB pathway and selectively binds tumor necrosis factor receptor-associated factor 1. RELT/TNFRSF19L is able to activate the NF-kappaB pathway and selectively binds tumor necrosis factor receptor-associated factor 1. Although the soluble form of RELT fusion protein does not inhibit the one-way mixed lymphocyte reaction, immobilized RELT/TNFRSF19L is capable of costimulating T-cell proliferation in the presence of CD3 signaling.

This RELT/TNFRSF19L Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human RELT/TNFRSF19L. It contains Human RELT/TNFRSF19L capture antibody, Human RELT/TNFRSF19L detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human RELT/TNFRSF19L protein.

References

Sica GL, et al. (2001) RELT, a new member of the tumor necrosis factor receptor superfamily, is selectively expressed in hematopoietic tissues and activates transcription factor NF-kappaB. Blood. 97(9): 2702-7.Polek TC, et al. (2006) The TNF receptor, RELT, binds SPAK and uses it to mediate p38 and JNK activation. Biochem Biophys Res Commun. 343(1): 125-34.Cusick JK, et al. (2006) Identification of RELT homologues that associate with RELT and are phosphorylated by OSR1. Biochem Biophys Res Commun. 340(2): 535-43.