interferon-alpha/IFNA5 ELISA Pair Set | IFNA5 elisa pair set

interferon-alpha/IFNA5 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118235

• Synonyms: interferon-alpha/IFNA5; interferon-alpha/IFNA5 Matched ELISA Antibody Pair Set; Human; Human IFNA5/IFNaG ELISA Pair Set; interferon; alpha 5; IFN-alpha Matched ELISA Antibody Pair Set; IFN-alpha-5 Matched ELISA Antibody Pair Set; IFN-alphaG Matched ELISA Antibody Pair Set; INA5 Matched ELISA Antibody Pair Set; INFA5 Matched ELISA Antibody Pair Set; leIF G Matched ELISA Antibody Pair Set; RP11-380P16.5 Matched ELISA Antibody Pair Set; IFNA5 elisa pair set

• Specificity: Quantitative determination of Human interferon-alpha/IFNA5

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 39.06-2500pg/mL
• Sensitivity: 39.06pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human IFNA5/IFNaG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human IFNA5/IFNaG polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 90 ng of recombinant Human IFNA5/IFNaG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for IFNA5 elisa pair set

Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

This interferon-alpha/IFNA5 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human interferon-alpha/IFNA5. It contains Human interferon-alpha/IFNA5 capture antibody, Human interferon-alpha/IFNA5 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human interferon-alpha/IFNA5 protein.

References

Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.