Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissueAll lanes: CCR9 antibody at 1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43, 41 KDaObserved band size: 43 KDa)
CCR9 recombinant antibody
CCR9 Antibody
IF: 1:20-1:200
IP: 1:200-1:1000
Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissueAll lanes: CCR9 antibody at 1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43, 41 KDaObserved band size: 43 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissueAll lanes: CCR9 antibody at 1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43, 41 KDaObserved band size: 43 KDa)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-RA004848A0HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-RA004848A0HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunoprecipitation (IP)
(Immunoprecipitating CCR9 in HL-60 whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA004848A0HU in HL-60 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA004848A0HU (3ug) + HL-60 whole cell lysate (500ug)Lane 3: HL-60 whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating CCR9 in HL-60 whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA004848A0HU in HL-60 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA004848A0HU (3ug) + HL-60 whole cell lysate (500ug)Lane 3: HL-60 whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
Research Articles on CCR9
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Product Notes
The CCR9 ccr9 (Catalog #AAA7114793) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCR9 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCR9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:5000 IF: 1:20-1:200 IP: 1:200-1:1000. Researchers should empirically determine the suitability of the CCR9 ccr9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR9, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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