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Western Blot (WB) (Western Blot analysis of Mouse liver, Mouse serum with AFP Polyclonal Antibody.)

Endodermal Lineage Marker Antibody Kit

Endodermal Lineage Marker Antibody Sampler Kit

Applications
Western Blot
Synonyms
Endodermal Lineage Marker; Endodermal Lineage Marker Antibody Sampler Kit; Endodermal Lineage Marker antibody kit
Ordering
For Research Use Only!
Applicable Applications for Endodermal Lineage Marker antibody kit
Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of Mouse liver, Mouse serum with AFP Polyclonal Antibody.)

Western Blot (WB) (Western Blot analysis of Mouse liver, Mouse serum with AFP Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of HT29 cells with EOMES Polyclonal Antibody.)

Western Blot (WB) (Western Blot analysis of HT29 cells with EOMES Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of Lovo cell using FOXA2 Polyclonal Antibody at dilution of 1:500)

Western Blot (WB) (Western Blot analysis of Lovo cell using FOXA2 Polyclonal Antibody at dilution of 1:500)

Western Blot (WB)

(Western Blot analysis of HT29 cells using HNF-4alpha Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB) (Western Blot analysis of HT29 cells using HNF-4alpha Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of various cells using N-cadherin Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB) (Western Blot analysis of various cells using N-cadherin Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells with PDGFR-alpha Polyclonal Antibody.)

Western Blot (WB) (Western Blot analysis of HepG2 cells with PDGFR-alpha Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of K-562 cells using SALL4 Monoclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of K-562 cells using SALL4 Monoclonal Antibody at dilution of 1:1000)
Related Product Information for Endodermal Lineage Marker antibody kit
Background: Two endodermal lineages develop during mammalian embryogenesis, the primitive endoderm of the blastocyst stage embryo and the definitive endoderm at gastrulation. The primitive endoderm gives rise to extra- embryonic lineages encompassing the visceral and the parietal endoderm. The definitive endoderm contributes to the respiratory and gastrointestinal tracts by forming the epithelial lining of the trachea, esophagus, lungs, stomach and intestines, and is a major component of many glands, including thyroid, thymus, pancreas and liver. Understanding molecular mechanisms that regulate early endodermal fates is seminal for the advance of stem cell research as they connect the transition from pluripotency to endoderm specification during mammalian development and contribute to the generation of clinically relevant cell types. FoxA2/HNF3beta is a transcription factor essential for development of the endoderm and midline structures in mouse embryos. EOMES acts during gastrulation to promote the specification of the definitive endoderm. Markers of hepatic differentiation in the endoderm include expression of alpha-fetoprotein (AFP) and N-cadherin. HNF4alpha is involved in the differentiation of the visceral endoderm. GATA-6 lies upstream of HNF4 in a transcriptional cascade that regulates differentiation of the visceral endoderm and is also required for the establishment of the endodermally derived bronchial epithelium. Sall4 is required for the formation of the primitive endoderm from inner cell mass. It has been reported that extra-embryonic stem cell lines cannot be formed in Sall4-deficient blastocysts. PDGF receptor alpha is expressed in primitive endoderm derivatives throughout embryogenesis.

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Product Notes

The Endodermal Lineage Marker (Catalog #AAA2550137) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Endodermal Lineage Marker can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Endodermal Lineage Marker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endodermal Lineage Marker, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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