Procaspase Antibody Kit

Procaspase Antibody Sampler Kit

Cat. Num. AAA2550013

• Applications: Western Blot
• Synonyms: Procaspase; Procaspase Antibody Sampler Kit; Procaspase antibody kit

• Applicable Applications for Procaspase antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of KB, MCF7 cells using Caspase-3 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells using Caspase-6 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of HT29 cells using Caspase-7 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of 3T3 cells with Caspase-8 Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of various cells using Caspase-9 Polyclonal Antibody at dilution of 1:500.)

Western Blot (WB)

(Western Blot analysis of 3T3 cells using Lamin A/C Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of Rat musle using PARP-1 Polyclonal Antibody at dilution of 1:1000.)

Related Product Information for Procaspase antibody kit

Background: Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 2, 8, 9, 10 and 12) are closely coupled to proapoptotic signals, which include the FasL, TNF-alpha, and DNA damage. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, alpha-fodrin, DFF and lamin A, and induce apoptosis. Caspase-8 (FLICE, Mch5, MACH) and Caspase-9 (ICE-LAP6, Mch6) are initiator caspases. CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activate caspase-8, leading to the release of the caspase-8 active fragments, p18 and p10. Cytochrome c released from the mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Caspase-3 (CPP-32, Apoptain, Yama, SCA-1), Caspase-6 (Mch2), and Caspase-7 (CMH-1, Mch3, ICE-LAP3) are effector caspases. Activation of caspase-3 requires proteolytic processing of its inactive zymogen/proform into activated p17 and p12 subunits. Procaspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits. Procaspase-6 is cleaved by caspase-3 at Asp23, Asp179 and Asp193 to form active large (p18) and small (p11) subunits. PARP, a 116 kDa nuclear poly (ADP- ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization. Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into large (41-50 kDa) and small (28 kDa) fragments. The cleavage of lamins results in nuclear disregulation and cell death.