Adipogenesis Marker Antibody Kit

Adipogenesis Marker Antibody Sampler Kit

Cat. Num. AAA2549970

• Applications: Western Blot
• Synonyms: Adipogenesis Marker; Adipogenesis Marker Antibody Sampler Kit; Adipogenesis Marker antibody kit

• Applicable Applications for Adipogenesis Marker antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of Hela cells with Phospho-ACCalpha (Ser80) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 823-AV cells using ACCalpha Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of Hela cells using Acrp30 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of 293T cells with C/EBP alpha Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells with Phospho-C/EBP alpha (Thr230) Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells with Phospho-C/EBP alpha (Ser21) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of A549 cells using Fatty Acid Synthase Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of Human fat tissue using PLIN1 Polyclonal Antibody at dilution of 1:250)

Western Blot (WB)

(Western Blot analysis of HuvEc cells using PPAR-gamma Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of PC-3 cells with Phospho-PPAR-gamma (Ser112) Polyclonal Antibody at dilution of 1:500)

Related Product Information for Adipogenesis Marker antibody kit

Background: Adipocytes are the primary cellular component of adipose tissue and play a key role in the storage of triacylglycerol. Adipogenesis is the cellular process where preadipocytes differentiate into adipocytes. Fatty acid binding proteins (FABPs) act as cytoplasmic lipid chaperones by binding fatty acids and lipids for transport to various cellular pathways. The predominant fatty acid binding protein found in adipocytes is FABP4. Adiponectin is an adipokine expressed exclusively in brown and white adipocytes and is secreted into the blood. It exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer. Decreased adiponectin levels are seen in obese and insulin-resistant mice and humans, suggesting that this adipokine is critical for maintenance of insulin sensitivity. Peroxisome proliferator-activated receptor gamma (PPARgamma) is a transcriptional activator preferentially expressed in adipocytes, vascular smooth muscle cells, and macrophages. Acetyl-CoA carboxylase (ACC) is a key fatty acid biosynthesis and oxidation enzyme that is responsible for the carboxylation of acetyl-CoA to malonyl-CoA. Phosphorylation of acetyl- CoA carboxylase by AMPK at Ser79 or by PKA at Ser1200 inhibits ACC enzymatic activity. ACC is a potential target of anti-obesity drugs. CCAAT/enhancer-binding proteins (C/EBPs) transcription factors are critical for cellular differentiation, terminal function, and the inflammatory response. Phosphorylation of C/EBPalpha at Thr222, Thr226, and Ser230 by GSK-3 may be required for adipogenesis. Perilipin localizes to the periphery of lipid droplets and serves as a protective coating against lipases. Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin, resulting in a conformational change that exposes lipid droplets to endogenous, hormone-sensitive lipases. Hence, perilipin plays a pivotal role in lipid storage. Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FASN is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FASN is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis.