Western Blot (WB)
(Western Blot analysis of HT-29 cell using HMGA1 Polyclonal Antibody at dilution of 1/250)
High Mobility Group (HMG) Proteins Antibody Kit | HMG antibody kit
High Mobility Group (HMG) Proteins Antibody Sampler Kit
Gene Names
HMG; ATHMG; high mobility group; NFD; NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR D; SSRP1
Applications
Western Blot
Synonyms
High Mobility Group (HMG) Proteins; High Mobility Group (HMG) Proteins Antibody Sampler Kit; HMG antibody kit
Sequence Length
646
Applicable Applications for HMG antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of HT-29 cell using HMGA1 Polyclonal Antibody at dilution of 1/250)
Western Blot (WB)
(Western Blot analysis of HT-29 cell using HMGA1 Polyclonal Antibody at dilution of 1/250)
Western Blot (WB)
(Western Blot analysis of Jurkat, K562 cells using HMG-1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Jurkat, K562 cells using HMG-1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with HMG-2 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with HMG-2 Polyclonal Antibody.)
Related Product Information for HMG antibody kit
Background: High mobility group (HMG) proteins are a superfamily of abundant and ubiquitous nuclear proteins that bind DNA without sequence specificity and induce structural changes to the chromatin fiber to regulate access to the underlying DNA. HMGA1, formerly known as HMG-I/Y, belongs to a family of high mobility group proteins known as HMGA. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors. HMGA1 is highly expressed during embryogenesis and in embryonic stem cells, but not in fully differentiated adult tissues. High mobility group protein B1 (HMGB1) and high mobility group protein B2 (HMGB2) belong to a family of highly conserved proteins that contain HMG box domains. HMGB1 is a widely expressed and highly abundant protein. HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals. While expression varies, the biochemical properties of the different family members may be indistinguishable. HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters. In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis, but not apoptosis. Extracellular HMGB1 "alarms" the innate immune system by acting as a chemoattractant for inflammatory cells triggering activation of T cells and dendritic cells. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation end products (RAGE). The HMGN family of proteins, which includes five members (HMGN1-5) function in transcriptional regulation and are recruited to gene promoters by transcription factors, such as estrogen receptor alpha (ERalpha), serum responsive factor (SRF), and PITX2, where they can facilitate either gene activation or repression. The expression of HMGN1 (also known as HMG14) and HMGN2 (also known as HMG17) is tightly linked to cellular differentiation. HMGN1 and HMNG2 are ubiquitous and highly expressed in all embryonic tissues. During mouse embryogenesis, expression is down-regulated throughout the embryo, except in committed but continuously renewing cell types undergoing active differentiation, such as the basal layer of the epithelium and kidney cells undergoing mesenchyme to epithelium transition.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
high mobility group
NCBI Official Symbol
HMG
NCBI Official Synonym Symbols
ATHMG; high mobility group; NFD; NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR D; SSRP1
NCBI Protein Information
high mobility group
Protein Family
NCBI Description
encodes a component of the FAcilitates Chromatin Transcription (FACT) complex, SSRP1. Along with STP16 binds to the promoter of FLC.
Research Articles on HMG
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Product Notes
The HMG (Catalog #AAA2550146) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's High Mobility Group (HMG) Proteins can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the HMG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "High Mobility Group (HMG) Proteins, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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