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Western Blot (WB)
(Western Blot analysis of HT29 cells using PKM2 Polyclonal Antibody at dilution of 1:2000.)
Glycolysis Antibody Kit | tpiA antibody kit
Glycolysis Antibody Sampler Kit
Applications
Western Blot
Synonyms
Glycolysis; Glycolysis Antibody Sampler Kit; tpiA antibody kit
Sequence Length
252
Applicable Applications for tpiA antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of HT29 cells using PKM2 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of HT29 cells using PKM2 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 293T, Rat brain, NIH, 3T3, Sheep muscle, Rabbit testis using GAPDH Polyclonal Antibody at dilution of 1:5000.)
Western Blot (WB)
(Western Blot analysis of 293T, Rat brain, NIH, 3T3, Sheep muscle, Rabbit testis using GAPDH Polyclonal Antibody at dilution of 1:5000.)
Western Blot (WB)
(Western Blot analysis of A549 cells using PDHA1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of A549 cells using PDHA1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using HXK I Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using HXK I Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of A431, K562, 231, hela and hepG2 cell using HK2 Polyclonal Antibody at dilution of 1:1200)
Western Blot (WB)
(Western Blot analysis of A431, K562, 231, hela and hepG2 cell using HK2 Polyclonal Antibody at dilution of 1:1200)
Western Blot (WB)
(Western Blot analysis of pig liver tissue using LDHA Monoclonal Antibody at dilution of 1:1500)
Western Blot (WB)
(Western Blot analysis of pig liver tissue using LDHA Monoclonal Antibody at dilution of 1:1500)
Western Blot (WB)
(Western Blot analysis of Jurkat cells with PFK-C Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Jurkat cells with PFK-C Polyclonal Antibody.)
Related Product Information for tpiA antibody kit
Background: Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate. Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development. Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production. The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
26,827 Da
NCBI Official Full Name
Glycolysis
UniProt Protein Name
Triosephosphate isomerase
Protein Family
UniProt Gene Name
tpiA
UniProt Synonym Gene Names
TIM; TPI
Uniprot Description
Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).
Similar Products
Product Notes
The Glycolysis tpia (Catalog #AAA2550037) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glycolysis can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Glycolysis tpia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycolysis, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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