Sheep anti-Human TAFA2 Polyclonal Antibody

TAFA2 (FAM19A2, Protein FAM19A2, Chemokine-like Protein TAFA-2, FAM19A2)

Cat. Num. AAA643226

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TAFA2; Polyclonal Antibody; TAFA2 (FAM19A2; Protein FAM19A2; Chemokine-like Protein TAFA-2; FAM19A2); Anti -TAFA2 (FAM19A2; anti-TAFA2 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAFA2. Species sequence homology: Human TAFA3 and rhTAFA4 (10%), rhTAFA5 (2%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.

• Applicable Applications for anti-TAFA2 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry and Neutralization.; Dilution: Immunohistochemistry: 5-15ug/ml
• Immunogen: Recombinant corresponding to aa31-131 from human TAFA2/FAM19A2 expressed in NS0 cells (Q8N3H0).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(TAFA2/FAM19A2 in Human Brain. TAFA2/FAM19A2 was detected in immersion fixed paraffin­embedded sections of human brain (cortex) using Human TAFA2/FAM19A2 Antigen Affinity­purified Polyclonal Antibody at 10ug/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat­induced epitope retrieval using Antigen Retrieval Reagent­Basic. Tissue was stained using the Sheep HRP­DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin­ embedded Tissue Sections.)

Related Product Information for anti-TAFA2 antibody

TAFA2 is a secreted, 11kD member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 131aa precursor that contains a 30aa signal sequence and a 101 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX7CCX13CXCX14CX11CX4CX5CX10C, where C represents a conserved cysteine residue andxrepresents any noncysteine aa (1). Human TAFA2 is 97aa identical to mouse TAFA2 (1). TAFA2 expression can be detected in the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus, but its expression in the CNS is 50- to 1000 -fold higher than in other tissues (1). Within the CNS, TAFA2 expression is highest in the occipital and frontal cortex (3- to 10 -fold more abundantly expressed than in other cortical regions) and medulla (1). The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1-2). Finally, TAFAs may control axonal sprouting following brain injury.

Product Categories/Family for anti-TAFA2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 32967229
• NCBI Official Full Name: TAFA2

Uniprot Description

FAM19A2: a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to MIP-1alpha, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines, that act as regulators of immune and nervous cells. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: cytoplasm