Rabbit anti-Mouse Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) Polyclonal Antibody | anti-XIRP1 antibody

Anti-Phospho-Ser295 Xin Actin-Binding Repeat-Containing Protein1 (XIRP1)

Cat. Num. AAA502180

• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295); Polyclonal Antibody; Anti-Phospho-Ser295 Xin Actin-Binding Repeat-Containing Protein1 (XIRP1); anti-XIRP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~250k and ~150k splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling of the XIRP1 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10- mini Western Blots.
• Sequence Length: 1129

• Applicable Applications for anti-XIRP1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser295 of mouse XIRP1.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH
• Immunogen Species: Mouse
• Reactivity Assumed Based on 100% Sequence Homology: Canine, human, non-human primate, rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will react with canine, human, non-human primate, and rat tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with beta-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXinalpha (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser295 is thought to play a key role in XIRP signaling.
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-XIRP1 antibody

Affinity purified rabbit polyclonal antibody

References

• Choi S, Gustafson-Wagner EA, Wang Q, Harlan SM, Sinn HW, Lin J.L.-C., Lin, J.J.-C. The Intercalated Disc Protein, mXinalpha, Is Capable of Interacting with beta-Catenin and Bundling Actin Filaments. J Biol Chem. 2007; 282(49):36024-36036.
• Gustafson-Wagner EA, Sinn H.W., Chen Y.-L., Wang D.-Z., Reiter R.S., Lin J.L.-C., Yang B., Williamson R.A., Chen J., Lin C.-I., Lin J.J.-C. Loss of mXinalpha, an intercalated disk protein, results in cardiac hypertrophy and cardiomyopathy with conduction defects. (2007) Am J Physiol Heart Circ Physiol, November; 293(5): H2680-H2692.
• Grosskurth SE, Bhattacharya D, Wang Q, Lin J.J.-C. Emergence of Xin Demarcates a Key Innovation in Heart Evolution. (2008) PLoS One. Aug; 3(8):e2857

NCBI and Uniprot Product Information

• NCBI GI #: 81870491
• UniProt Accession #: O70373
• Molecular Weight: 150, 250
• NCBI Official Full Name: Xin actin-binding repeat-containing protein 1
• UniProt Protein Name: Xin actin-binding repeat-containing protein 1
• Protein Family: Xin actin-binding repeat-containing protein
• UniProt Gene Name: Xirp1
• UniProt Synonym Gene Names: Cmya1; Xin
• UniProt Entry Name: XIRP1_MOUSE

Uniprot Description

Function: Protects actin filaments from depolymerization

By similarity.

Subunit structure: Interacts with FLNC and VASP

By similarity. Interacts with F-actin and CTNNB1. Ref.6

Subcellular location: Cell junction

By similarity. Note: Colocalizes with actin stress fibers

By similarity.

Tissue specificity: Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level). Ref.1 Ref.5

Developmental stage: At E8, expression is restricted to cardiogenic cells. At E10, expressed in heart and rostral somites. At E13.5, expressed in heart and skeletal muscle (at protein level). Ref.4

Domain: Xin repeats bind F-actin.

Disruption phenotype: Mice are predisposed to hypertrophic cardiomyopathy, and display abnormal cardiac electrophysiological characteristics, including defects in atrial depolarization and ventricular repolarization. Ref.5

Sequence similarities: Belongs to the Xin family.Contains 17 Xin repeats.