Rabbit anti-Rat Clavesin Polyclonal Antibody | anti-CLVS1/2 antibody

Anti-Clavesin 1/2

Cat. Num. AAA502146

• Gene Names: Clvs1; Rlbp1l1; Clavesin-1; RGD1564200
• Reactivity: Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified (Prepared from serum by affinity purification using a column to which the peptide antigen was coupled.)
• Synonyms: Clavesin; Polyclonal Antibody; Anti-Clavesin 1/2; anti-CLVS1/2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~ 35 kDa clavesin 1/2 protein doublet in Western blots of rat brain lysate. Isoform-specific knock down in cultured hippocampal neurons indicates that the lower and upper bands are clavesin 1 and 2, respectively.
• Purity/Purification: Affinity Purified (Prepared from serum by affinity purification using a column to which the peptide antigen was coupled.)
• Form/Format: 100 ul in PBS + 50% glycerol
• Sequence Length: 354

• Applicable Applications for anti-CLVS1/2 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1,000; ICC: 1:50
• Antigen: Synthetic peptide taken from the C-terminus of rat clavesin 1.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Human, mouse
• Species Reactivity Note: The antibody has been directly tested for reactivity in rat. It is anticipated that the antibody will also work in human and mouse as there is 100% homology in these species with the amino acid sequence used as antigen.
• Biological Significance: Clavesin (clathrin vesicle associated Sec14 protein) is a novel neuron specific protein that has recently been identified and shown to be required for normal morphology of late endosomes and/or lysosomes as lentiviral-mediated knockdown of clavesin in hippocampal neurons causes lysosomal defects (Katoh et al., 2009). Additionally, upregulation of clavesin in human hepatocellular carcinoma has recently been demonstrated thus making it a useful marker for this disease state (Zhao et al., 2008).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-CLVS1/2 antibody

Affinity purified rabbit polyclonal antibody

References

• Yohei Katoh, Brigitte Ritter, Thomas Gaffry, Francois Blondeau, Stefan Höning and Peter S. McPherson (2009) the clavesin family: neuron-specific lipid- and clathrin-binding Sec14 proteins regulating lysosomal morphology. Journal of Biological Chemistry Oct 2;284(40):27646-54.
• Zhao S, Xu C, Qian H, Lv L, Ji C, Chen C, Zhao X, Zheng D, Gu S, Xie Y, Mao Y (2008) Cellular retinaldehyde-binding protein-like (CRALBPL), a novel human Sec14p-like gene that is upregulated in human hepatocellular carcinomas, may be used as a marker for human hepatocellular carcinomas. DNA Cell Biol. Mar; 27(3):159-63.

NCBI and Uniprot Product Information

• NCBI GI #: 157822747
• NCBI GeneID: 366311
• NCBI Accession #: NP_001102439.1
• NCBI GenBank Nucleotide #: NM_001108969.1
• UniProt Accession #: A6JFQ6
• Molecular Weight: 35
• NCBI Official Full Name: clavesin-1
• NCBI Official Synonym Full Names: clavesin 1
• NCBI Official Symbol: Clvs1
• NCBI Official Synonym Symbols: Rlbp1l1; Clavesin-1; RGD1564200
• NCBI Protein Information: clavesin-1; retinaldehyde-binding protein 1-like 1; Retinaldehyde-binding protein 1-like protein 1
• UniProt Protein Name: Clavesin-1
• Protein Family: Clavesin
• UniProt Gene Name: Clvs1
• UniProt Synonym Gene Names: Rlbp1l1
• UniProt Entry Name: CLVS1_RAT

Uniprot Description

Function: Required for normal morphology of late endosomes and/or lysosomes in neurons. Binds phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Ref.2 UniProtKB Q8IUQ0

Subunit structure: Forms a complex with clathrin heavy chain and gamma-adaptin

By similarity. UniProtKB Q8IUQ0

Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein

By similarity. Cytoplasmic vesicle › clathrin-coated vesicle Ref.2 UniProtKB Q8IUQ0.

Tissue specificity: Expressed in brain with no expression detected in non-neuronal tissues (at protein level). Ref.2

Domain: The CRAL-TRIO domain is required for targeting to the membrane and for binding PtdIns(3,5)P2

By similarity. UniProtKB Q8IUQ0

Miscellaneous: Binding to PtdIns(3,5)P2 is not required for localization

By similarity. UniProtKB Q8IUQ0

Sequence similarities: Contains 1 CRAL-TRIO domain.