Rabbit anti-Rat GRASP Polyclonal Antibody | anti-GRIPAP1 antibody

Anti-GRASP

Cat. Num. AAA502068

• Gene Names: Gripap1; Grasp1
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the C-terminal peptide used as antigen.)
• Synonyms: GRASP; Polyclonal Antibody; Anti-GRASP; anti-GRIPAP1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~95k GRASP protein.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the C-terminal peptide used as antigen.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 837

• Applicable Applications for anti-GRIPAP1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Peptide corresponding to amino acid residues from the C-terminal region of rat GRASP, conjugated to keyhole limpet hemocyanin (KLH).
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human and mouse
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: PDZ domain-containing proteins, such as PSD-95 and GRIP are thought to play key roles in glutamate receptor plasticity. GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within GRIP also play key roles in regulation of glutamate receptor function. GRASP-1 is a neuronal rasGEF associated with GRIP and AMPA receptors in vivo (Scannevin and Huganir, 2000). Recent work suggests that GRASP-1 may regulate neuronal ras signaling and contribute to the regulation of AMPA receptor distribution by NMDA receptor activity (Ye et al., 2000).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-GRIPAP1 antibody

Affinity purified rabbit polyclonal antibody

References

• Scannevin RH, Huganir RL (2000) Postsynaptic organization and regulation of excitatory synapses. Nat Rev Neurosci 1:133-141.
• Ye B, Liao DZ, Zhang XQ, Zhang PS, Dong HL, Huganir RL (2000) GRASP-1: A neuronal RasGEF associated with the AMPA receptor/GRIP complex. Neuron 26:603-617.

NCBI and Uniprot Product Information

• NCBI GI #: 16758652
• NCBI GeneID: 116493
• NCBI Accession #: NP_446259.1
• NCBI GenBank Nucleotide #: NM_053807.1
• UniProt Accession #: Q9JHZ4; Q9JHZ3
• Molecular Weight: 95
• NCBI Official Full Name: GRIP1-associated protein 1
• NCBI Official Synonym Full Names: GRIP1 associated protein 1
• NCBI Official Symbol: Gripap1
• NCBI Official Synonym Symbols: Grasp1
• NCBI Protein Information: GRIP1-associated protein 1; GRASP-1; GRIP-associated protein 1
• UniProt Protein Name: GRIP1-associated protein 1
• Protein Family: GRASP65 homolog protein
• UniProt Gene Name: Gripap1
• UniProt Synonym Gene Names: Grasp1; GRASP-1
• UniProt Entry Name: GRAP1_RAT

NCBI Description

a neuronal rasGEF that may regulate neuronal ras signaling and contribute to the regulation of AMPA receptor distribution by NMDA receptor activity [RGD, Feb 2006]

Uniprot Description

Subunit structure: Interacts with GRIP1, GRIP2 and AMPA receptors. Ref.1

Subcellular location: Early endosome

By similarity.

Tissue specificity: Expressed in the central nervous system; especially in neurons. Ref.1

Post-translational modification: Proteolytically cleaved by caspase-3. A minor C-terminal proteolytic fragment of 30 kDa is produced. Ref.1