Rabbit anti-Rat synaptophysin 2 Polyclonal Antibody | anti-SYNPR antibody

Anti-Synaptophysin 2

Cat. Num. AAA502121

• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.)
• Synonyms: synaptophysin 2; Polyclonal Antibody; Anti-Synaptophysin 2; anti-SYNPR antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for ~38k Synaptophysin 2 protein.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 265

• Applicable Applications for anti-SYNPR antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Peptide corresponding to amino acid residues from the C-terminal region of rat Synaptophysin 2, conjugated to keyhole limpet hemocyanin (KLH).
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Mouse
• Species Reactivity Note: The antibody has been demonstrated to work in rat. It is anticipated that the antibody will work in mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Synaptophysin 2, also known as Synaptoporin, is a component of the synaptic vesicle membrane and thought to play an important role in synaptic vesicle trafficking (Dai et al., 2003). It is highly homologous to Synaptophysin 1, yet differs in expression. Whereas Synaptophysin 1 is ubiquitiously expressed, Synaptophysin 2 is concentrated in the mossy fiber synapses of the hippocampus (Grabs et al., 1994; Singec et al., 2002).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-SYNPR antibody

Affinity purified rabbit polyclonal antibody

References

• Dai J, Ji C, Gu S, Wu Q, Wang L, Xu J, Zeng L, Ye X, Yin G, Xie Y, Mao Y (2003) Cloning and sequence analysis of the human cDNA encoding the synaptoporin, a highly conserved synaptic vesicle protein. Molecular Biology Reports 30:185-191
• Singec I, Knoth R, Ditter M, Hagemeyer CE, Rosenbrock H, Frotscher M, Volk B (2002) Synaptic vesicle protein synaptoporin is differently expressed by subpopulations of mouse hippocampal neurons. J Comp Neurol 452(2):139-153
• Grabs D, Bergmann M, Schuster T, Fox PA, Brich M, Gratz M (1994) Differential expression of synaptophysin and synaptoporin during pre- and postnatal development of the rat hippocampal network. Eur J Neurosci. 6(11): 1765-71..

NCBI and Uniprot Product Information

• NCBI GI #: 13027428
• NCBI GeneID: 66030
• NCBI Accession #: NP_076464.1
• NCBI GenBank Nucleotide #: NM_023974.1
• UniProt Accession #: P22831; Q9ERH1
• Molecular Weight: 38
• NCBI Official Full Name: synaptoporin
• NCBI Official Synonym Full Names: synaptoporin
• NCBI Official Symbol: Synpr
• NCBI Protein Information: synaptoporin; synaptorin
• UniProt Protein Name: Synaptoporin
• Protein Family: Synaptoporin
• UniProt Gene Name: Synpr
• UniProt Entry Name: SYNPR_RAT

NCBI Description

synaptic vesicle membrane protein; member of the synaptophysin family [RGD, Feb 2006]

Uniprot Description

Function: Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein.

Subcellular location: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein. Cell junction › synapse › synaptosome.

Tissue specificity: Central nervous system.

Sequence similarities: Belongs to the synaptophysin/synaptobrevin family.Contains 1 MARVEL domain.