Rabbit anti-Rat Synapsin (Ser549) Polyclonal Antibody | anti-SYN1 antibody

Anti-Phospho-Ser549 Synapsin I

Cat. Num. AAA502115

• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Synonyms: Synapsin (Ser549); Polyclonal Antibody; Anti-Phospho-Ser549 Synapsin I; anti-SYN1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for ~78k synapsin I doublet phosphorylated at Ser549. Immunolabeling of the synapsin I band is blocked by lambda-phosphatase treatment.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 706

• Applicable Applications for anti-SYN1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:500
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser549 of synapsin I.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, mouse, and non-human primate
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse, non-human primate, bovine and canine tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking.
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-SYN1 antibody

Affinity purified rabbit polyclonal antibody

References

• Jovanovic JN, Benfenati, F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P,Czernik AJ (1996) Neurotrophins stimulate phosphorylation of Synapsin I by MAP kinase and regulate Synapsin I-actin interactions. Neurobiology 93:3679-3683.
• Czernik AJ, Pang DT, Greengard P (1987) Amino acid sequences surrounding the cAMP-dependent and calcium/calmodulin-dependent phosphorylation sites in rat and bovine synapsin I. Proc Natl Acad Sci (USA) 84:7518-7522. Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380.
• Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953.
• Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.
• Nayak AS, Moore CI, Browning MD (1996) CAM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456.

NCBI and Uniprot Product Information

• NCBI GI #: 27806393
• NCBI GeneID: 281510
• NCBI Accession #: NP_776616.1
• NCBI GenBank Nucleotide #: NM_174191.2
• UniProt Accession #: P17599
• Molecular Weight: 78
• NCBI Official Full Name: synapsin-1
• NCBI Official Symbol: SYN1
• NCBI Protein Information: synapsin-1
• UniProt Protein Name: Synapsin-1
• Protein Family: Synapsin
• UniProt Gene Name: SYN1
• UniProt Entry Name: SYN1_BOVIN

Uniprot Description

Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level

By similarity.

Subunit structure: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ibinteracts with PRNP

By similarity.

Subcellular location: Cell junction › synapse. Golgi apparatus

By similarity.

Domain: The A region binds phospholipids with a preference for negatively charged species

By similarity.

Post-translational modification: Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles

By similarity.

Sequence similarities: Belongs to the synapsin family.