Rabbit anti-Rat Rabphilin 3A Polyclonal Antibody | anti-RPH3A antibody

Anti-Rabphilin 3A

Cat. Num. AAA502108

• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification on a column to which the C-terminal peptide immunogen was coupled.)
• Synonyms: Rabphilin 3A; Polyclonal Antibody; Anti-Rabphilin 3A; anti-RPH3A antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~82k rabphilin 3A protein
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification on a column to which the C-terminal peptide immunogen was coupled.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 684

• Applicable Applications for anti-RPH3A antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Synthetic peptide corresponding to amino acid residues from the C-terminal region of rabphilin 3A.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Mouse, human, bovine, canine and non-human primate
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, bovine, canine and non-human primate tissue based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-RPH3A antibody

Affinity purified rabbit polyclonal antibody

References

• Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.
• Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. J Neurosci 15:2385-2395.
• Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, Südhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond) 369:493-497.
• Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman ER, Jahn R, De Camilli P, Südhof TC (1994) Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid- binding protein, depends on rab3A/3C. Neuron 13:885-898.
• Smith R, Petersen A, Bates GP, Brundin P, Li JY (2005) Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington's disease, a possible culprit in synaptic dysfunction. Neurobiol Dis 20:673-684.

NCBI and Uniprot Product Information

• NCBI GI #: 19424162
• NCBI GeneID: 171039
• NCBI Accession #: NP_598202.1
• NCBI GenBank Nucleotide #: NM_133518.1
• UniProt Accession #: P47709
• Molecular Weight: 82
• NCBI Official Full Name: rabphilin-3A
• NCBI Official Synonym Full Names: rabphilin 3A
• NCBI Official Symbol: Rph3a
• NCBI Protein Information: rabphilin-3A; exophilin-1; rabphilin 3A homolog
• UniProt Protein Name: Rabphilin-3A
• Protein Family: Rabphilin
• UniProt Gene Name: Rph3a
• UniProt Entry Name: RP3A_RAT

NCBI Description

binds Ca2+ and phospholipids [RGD, Feb 2006]

Uniprot Description

Function: Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Subunit structure: Interacts with RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B

By similarity. Interacts with RAB3A. Monomer.

Subcellular location: Cell junction › synapse

By similarity. Membrane; Peripheral membrane protein Ref.4.

Tissue specificity: Specifically expressed in brain.

Domain: Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers. Ref.4

Sequence similarities: Contains 2 C2 domains.Contains 1 FYVE-type zinc finger.Contains 1 RabBD (Rab-binding) domain.