Rabbit anti-Rat Connexin 43 Polyclonal Antibody | anti-GJA1 antibody

Anti-Connexin43

Cat. Num. AAA502014

• Gene Names: Gja1; Cx43
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.)
• Synonyms: Connexin 43; Polyclonal Antibody; Anti-Connexin43; anti-GJA1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~43k connexin43 protein.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 382

• Applicable Applications for anti-GJA1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: Not recommended
• Antigen: Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Chicken, canine, finch, human, mouse non-human primate, sheep Xenopus and zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-GJA1 antibody

Affinity purified rabbit polyclonal antibody

References

• Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
• Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
• Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.
• Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136.

NCBI and Uniprot Product Information

• NCBI GI #: 6978896
• NCBI GeneID: 24392
• NCBI Accession #: NP_036699.1
• NCBI GenBank Nucleotide #: NM_012567.2
• UniProt Accession #: P08050; Q53ZE1
• Molecular Weight: 43
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 1
• NCBI Official Symbol: Gja1
• NCBI Official Synonym Symbols: Cx43
• NCBI Protein Information: gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1
• UniProt Protein Name: Gap junction alpha-1 protein
• Protein Family: Gap junction alpha-1 protein
• UniProt Gene Name: Gja1
• UniProt Synonym Gene Names: Cxn-43; Cx43
• UniProt Entry Name: CXA1_RAT

NCBI Description

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

Uniprot Description

Function: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract

By similarity.

Subunit structure: A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150. Interacts with CNST and CSNK1D

By similarity. Interacts (via C-terminus) with TJP1. Interacts (via C-terminus) with SRC (via SH3 domain). Interacts with UBQLN4. Ref.10 Ref.12 Ref.13

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction Ref.13.

Induction: In bladder smooth muscle cells, exhibits night/day variations with low levels during the sleep phase, at circadian time (CT) 4-8 (at protein level). Expression starts to increase around CT12 and forms a plateau during the active phase (CT16-24) (at protein level). Ref.11

Post-translational modification: Contains at least one intramolecular disulfide bond.Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity

By similarity. Ref.9Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2

By similarity.S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.

Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.