Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1) Recombinant Protein | FDFT1 recombinant protein

Recombinant Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1)

Cat. Num. AAA2012327

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1); Recombinant Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1); FDFT1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- FSAS EFEDPIVGED TECANSMGLF LQKTNIIRDY LEDQQEGRQF WPQEVWGKYV KKLEDFVKPE NVDVAVKCLN ELITNALQHI PDVITYLSRL RNQSVFNFCA IPQVMAIATL AACYNNHQVF KGVVKIRKGQ AVTLMMDATN MPAVKAIIYQ YIEEIYHRVP NSDPSASKAK QLISNIRTQS LPNCQLISRS HYSPIYLSFI MLLAALSWQY LSTLSQVTED YVQREH
• Sequence Length: 416

• Applicable Applications for FDFT1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Phe187~His416 (Accession # Q02769) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9506591
• NCBI GeneID: 29580
• NCBI Accession #: NP_062111.1
• NCBI GenBank Nucleotide #: NM_019238.2
• UniProt Accession #: Q02769
• Molecular Weight: 27.9kDa
• NCBI Official Full Name: squalene synthase
• NCBI Official Synonym Full Names: farnesyl diphosphate farnesyl transferase 1
• NCBI Official Symbol: Fdft1
• NCBI Protein Information: squalene synthase; SS; SQS; squalene synthetase; FPP:FPP farnesyltransferase; farnesyl diphosphate farnesyltransferase; farnesyl-diphosphate farnesyltransferase
• UniProt Protein Name: Squalene synthase
• UniProt Gene Name: Fdft1
• UniProt Synonym Gene Names: SQS; SS
• UniProt Entry Name: FDFT_RAT

NCBI Description

catalyzes the conversion of trans-farnesyl diphosphate to squalene; plays a role in biosynthesis of cholesterol and of isoprenoids [RGD, Feb 2006]

Uniprot Description

Function: Critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway.

Catalytic activity: 2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactor: Magnesium.

Pathway: Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.

Disruption phenotype: Hypomorphic mutations in the Lss gene and in this gene were identified, as well as a null mutation in the Lss gene. Cataract onset is associated with the specific combination of Lss and Fdft1 mutant alleles that decrease cholesterol levels in cataractous lenses to about 57% of normal. Cholesterol insufficiency may cause the deficient proliferation of lens epithelial cells in Shumiya cataract rats, resulting in the loss of homeostatic epithelial cell control of the underlying fiber cells and ultimately cataractogenesis. Ref.5

Sequence similarities: Belongs to the phytoene/squalene synthase family.