Connexin 43 (CX43) Recombinant Protein | CX43 recombinant protein

Recombinant Connexin 43 (CX43)

Cat. Num. AAA2011823

• Gene Names: Gja1; Cx43
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Connexin 43 (CX43); Recombinant Connexin 43 (CX43); CX43 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-S LSAVYTCKRD PCPHQVDCFL SRPTEKTIFI IFMLVVSLVS LALNIIELFY VFFKGVKDRV KGRSDPYHAT TGPLSPSKDC GSPKYAYFNG CSSPTAPLSP MSPPGYKLVT GDRNNSSCRN YNKQASEQNW ANYSAEQNRM GQAGSTISNS HAQPFDFPDD NQNAKKVAAG HELQPLAIVD QRPSSRASSR ASSRPRPDDL EI
• Sequence Length: 382

• Applicable Applications for CX43 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ser180~Ile382 (Accession # P08050) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6978896
• NCBI GeneID: 24392
• NCBI Accession #: NP_036699.1
• NCBI GenBank Nucleotide #: NM_012567.2
• UniProt Accession #: P08050; Q53ZE1
• Molecular Weight: 26.2kDa
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 1
• NCBI Official Symbol: Gja1
• NCBI Official Synonym Symbols: Cx43
• NCBI Protein Information: gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1
• UniProt Protein Name: Gap junction alpha-1 protein
• UniProt Gene Name: Gja1
• UniProt Synonym Gene Names: Cxn-43; Cx43
• UniProt Entry Name: CXA1_RAT

NCBI Description

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

Uniprot Description

GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Protein type: Channel, misc.; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: Golgi apparatus; contractile fiber; integral to plasma membrane; lysosome; early endosome; intercellular junction; fascia adherens; cytosol; lipid raft; connexon complex; multivesicular body; mitochondrial outer membrane; membrane; cytoplasm; apical plasma membrane; gap junction; lateral plasma membrane; endosome; endoplasmic reticulum membrane; focal adhesion; intermediate filament; Golgi membrane; late endosome; plasma membrane; cell junction

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; ion transmembrane transporter activity; beta-tubulin binding; SH3 domain binding; gap junction channel activity; PDZ domain binding; receptor binding

Biological Process: lens development in camera-type eye; response to peptide hormone stimulus; apoptosis; heart development; neuron migration; milk ejection; signal transduction; positive regulation of vasodilation; regulation of heart contraction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; negative regulation of cardiac muscle cell proliferation; positive regulation of glomerular filtration; response to glucose stimulus; positive regulation of striated muscle development; cell communication; heart looping; transmembrane transport; ATP transport; adult heart development; intercellular junction assembly and maintenance; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; gap junction assembly; in utero embryonic development; epithelial cell maturation; regulation of bone remodeling; positive regulation of insulin secretion; skeletal muscle regeneration; regulation of calcium ion transport; vascular transport; protein oligomerization; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of endothelial cell proliferation; positive regulation of protein catabolic process; positive regulation of vasoconstriction; blood vessel morphogenesis; embryonic heart tube development; embryonic digit morphogenesis; regulation of bone mineralization; neurite morphogenesis; response to pH