Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Brain Tissue.)
Rabbit Connexin 43 (CX43) Polyclonal Antibody | anti-CX43 antibody
Polyclonal Antibody to Connexin 43 (CX43)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CX43, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CX43, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Rat Cardiac Muscle Tissue;Primary Ab: 10ug/ml Rabbit Anti-Rat CX43 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Rat Cardiac Muscle Tissue;Primary Ab: 10ug/ml Rabbit Anti-Rat CX43 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]
Uniprot Description
GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
Protein type: Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20q11
Cellular Component: apical plasma membrane; cell junction; connexin complex; contractile fiber; cytoplasm; cytosol; early endosome; endoplasmic reticulum membrane; endosome; extracellular exosome; fascia adherens; focal adhesion; gap junction; Golgi apparatus; Golgi membrane; integral component of plasma membrane; intercalated disc; intercellular junction; intermediate filament; late endosome; lateral plasma membrane; lysosome; membrane; membrane raft; mitochondrial outer membrane; mitochondrion; multivesicular body; plasma membrane; protein complex
Molecular Function: beta-tubulin binding; gap junction channel activity; ion transmembrane transporter activity; PDZ domain binding; protein binding; protein domain specific binding; receptor binding; SH3 domain binding; signal transducer activity
Biological Process: adult heart development; apoptosis; ATP transport; atrial ventricular junction remodeling; blood vessel morphogenesis; cell communication; cell communication by chemical coupling; cell communication by electrical coupling; cell-cell junction organization; cell-cell signaling; cellular response to parathyroid hormone stimulus; cellular response to pH; chronic inflammatory response; decidualization; elevation of cytosolic calcium ion concentration; embryonic digit morphogenesis; embryonic heart tube development; endothelium development; epithelial cell maturation; gap junction assembly; heart development; heart looping; in utero embryonic development; lens development in camera-type eye; milk ejection reflex; negative regulation of cardiac muscle cell proliferation; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of DNA biosynthetic process; negative regulation of endothelial cell proliferation; negative regulation of gene expression; neuron migration; neuron projection morphogenesis; osteoblast differentiation; positive regulation of behavioral fear response; positive regulation of blood vessel diameter; positive regulation of cell communication by chemical coupling; positive regulation of gene expression; positive regulation of glomerular filtration; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of insulin secretion; positive regulation of osteoblast differentiation; positive regulation of protein catabolic process; positive regulation of striated muscle tissue development; positive regulation of vasoconstriction; protein oligomerization; regulation of bicellular tight junction assembly; regulation of bone mineralization; regulation of bone remodeling; regulation of calcium ion transport; regulation of heart contraction; regulation of transmembrane transporter activity; response to glucose stimulus; response to lipopolysaccharide; response to peptide hormone; response to pH; response to retinoic acid; signal transduction; skeletal muscle tissue regeneration; transmembrane transport; vascular transport
Research Articles on CX43
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Product Notes
The CX43 gja1 (Catalog #AAA2003509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Connexin 43 (CX43) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 43 (CX43) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CX43 gja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 43 (CX43), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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