Rabbit anti-Rat Dynamin Polyclonal Antibody | anti-DNM1 antibody

Anti-Dynamin

Cat. Num. AAA502027

• Gene Names: DNM1; DNM
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.)
• Synonyms: Dynamin; Polyclonal Antibody; Anti-Dynamin; anti-DNM1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for ~95k dynamin protein.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 851

• Applicable Applications for anti-DNM1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Peptide corresponding to amino acid residues from the N-terminal region of human Dynamin, conjugated to keyhole limpet hemocyanin (KLH).
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
• Immunogen Species: Human
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, chicken, human, mouse and non-human primate
• Species Reactivity Note: The antibody has been demonstrated to work in rat. It is also anticipated that the antibody will work with human, mouse, non-human primates, cow and chicken based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). There are 3 known isofoms of Dynamin, each having several splice variants as well. Dynamin I is expressed only in neurons whereas Dynamin II is ubiquitously expressed and Dynamin III is found primarily in the testes. Dynamin 1 is phosphorylated by PKC and dephosphorylated by calcineurin.
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-DNM1 antibody

Affinity purified rabbit polyclonal antibody

References

• Cousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem 76:105-116.
• Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA 99:7124-7129.
• Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.
• Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A (2002) Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. J Biol Chem 277:15957-15961.

NCBI and Uniprot Product Information

• NCBI GI #: 56549117
• NCBI GeneID: 1759
• NCBI Accession #: NP_001005336.1
• NCBI GenBank Nucleotide #: NM_001005336.2
• UniProt Accession #: Q05193; Q5SYX0; Q5SYX2; Q6P3T6; Q86VD2; A6NLM6
• Molecular Weight: 95
• NCBI Official Full Name: dynamin-1 isoform 2
• NCBI Official Synonym Full Names: dynamin 1
• NCBI Official Symbol: DNM1
• NCBI Official Synonym Symbols: DNM
• NCBI Protein Information: dynamin-1
• UniProt Protein Name: Dynamin-1
• Protein Family: Dynamin
• UniProt Gene Name: DNM1
• UniProt Synonym Gene Names: DNM
• UniProt Entry Name: DYN1_HUMAN

NCBI Description

This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.

Catalytic activity: GTP + H2O = GDP + phosphate.

Subunit structure: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3

By similarity. Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3

By similarity. Interacts with SNX9. Interacts with MYO1E (via SH3 domain). Interacts with SNX33 (via SH3 domain). Ref.4 Ref.6 Ref.7

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Ref.4

Sequence similarities: Belongs to the dynamin family.Contains 1 GED domain.Contains 1 PH domain.