Mouse BoNT B, lightchain Monoclonal Antibody

anti-botulinum neurotoxin type B (BoNT/B) monoclonal antibody

Cat. Num. AAA468003

• Reactivity: Clostridium Botulinum
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: Protein G
• Synonyms: BoNT B; lightchain; Monoclonal Antibody; anti-botulinum neurotoxin type B (BoNT/B) monoclonal antibody; anti-BoNT B; lightchain antibody

• Host: Mouse
• Reactivity: Clostridium Botulinum
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: GR-3G7
• Specificity: anti-BoNT/B (Clostridium botulinum, serotype B, light chain)
• Purity/Purification: Protein G
• Form/Format: Purified

• Applicable Applications for anti-BoNT B, lightchain antibody: Flow Cytometry (FC/FACS), CELISA
• Application Notes: Flow Cytometry: 1.2 ug/10^6 cells; CELISA: 1:200 - 1:400
• Buffer: phosphate buffered saline, pH 7.2
• Immunogen: genetic immunisation with cDNA encoding BoNT/B
• Selection: based on recognition of the complete native protein expressed on transfected mammalian cells
• Immunogen: cDNA
• Preparation and Storage: Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)

Testing Data

(Fig.1: FACS analysis of BOSC23 cells using GR-3G7 Cat.# GM-0701. BOSC23 cells were transiently transfected with an expression vector encoding either BoNT/B (red curve) or an irrelevant protein (control transfectant). Binding of GR-3G7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with BoNT/B transfected cells.)

Testing Data #2

(Fig.2: BOSC23 cells were transiently transfected with expression vectors containing the cDNA of the light chain of botulinum toxin A-F. Expression of the constructs was tested with an anti-myc antibody (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GR-3G7 was tested on all botulinum toxin transfectants. A positive signal was obtained only with BoNT/B transfected cells (red curves).)

Testing Data #3

(SDS-PAGE analysis of GR-3G7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified GR- 3G7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GR-3G7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)

Related Product Information for anti-BoNT B, lightchain antibody

Background: Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C1,D,E,F, and G (1). BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release (2,3). BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment (4).

References

DasGupta BR (1990). Structure and biological activity of botulinum neurotoxin. J Physiol (Paris) 84(3):220- 8
Schiavo G, Benfenati F, Poulain B, Rossetto O, Polverino de Laureto P, DasGupta BR and Montecucco C (1992). Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin. Nature 29; 359(6398):832-5
Tonello F, Morante S, Rossetto O, Schiavo G and Montecucco C (1996). Tetanus and botulinum toxins: a novel group of zinc-endopeptidases. Adv Exp Med Biol 389:251-60
Papapetropoulos S and Singer C (2007). Botulinum toxin in movement disorders. Semin Neurol 27(2):183-94