Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-13C1 Cat.# GM-0301. BOSC23 cells were transiently transfected with an expression vector encoding either ICOSL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-13C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ICOSL transfected cells.)
Mouse anti-Human ICOSL Monoclonal Antibody | anti-ICOSLG antibody
anti-human Inducible co-stimulator ligand (ICOSL) monoclonal antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-13C1 Cat.# GM-0301. BOSC23 cells were transiently transfected with an expression vector encoding either ICOSL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-13C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ICOSL transfected cells.)
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-13C1 Cat.# GM-0301. BOSC23 cells were transiently transfected with an expression vector encoding either ICOSL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-13C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ICOSL transfected cells.)
Testing Data #2
(SDS-PAGE analysis of GM-13C1 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-13C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-13C1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Testing Data #2
(SDS-PAGE analysis of GM-13C1 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-13C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-13C1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Richter G and Burdach S (2004). ICOS: a new costimulatory ligand/receptor pair and its role in T-cell activation. Onkologie 27(1): 91-5
Aicher A, Hayden-Ledbetter M, Brady WA, Pezzutto A, Richer G, Magaletti D, Buckwalter S, Ledbetter JA and Clark EA (2000). Characterization of human inducible costimulator ligand expression and function. J Immunol 1;164(9):4689-96
NCBI and Uniprot Product Information
Uniprot Description
ICOSLG: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co- stimulating memory T-cell function. Belongs to the immunoglobulin superfamily. BTN/MOG family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: integral to membrane; plasma membrane
Molecular Function: receptor binding
Biological Process: hyperosmotic response; B cell activation; T cell activation; T cell costimulation; defense response; positive regulation of activated T cell proliferation; signal transduction
Research Articles on ICOSLG
Similar Products
Product Notes
The ICOSLG icoslg (Catalog #AAA468040) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Inducible co-stimulator ligand (ICOSL) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICOSL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS)and CELISA. Immunofluorescence: genetic immunisation with cDNA encoding human ICOSL (extracellular domain) Flow Cytometry: 1.2 ug/10^6 cells CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the ICOSLG icoslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICOSL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
