Testing Data
(Fig.1: FACS analysis of BOSC23 cells using BCI-3E7 Cat.# GM- 1005. BOSC23 cells were transiently transfected with an expression vector encoding either c-Met (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BCI-3E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with c-Met transfected cells.)
Rat anti-Human c-Met Monoclonal Antibody | anti-MET antibody
Anti-c Met monoclonal antibody
CELISA: 1:200 - 1:400
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using BCI-3E7 Cat.# GM- 1005. BOSC23 cells were transiently transfected with an expression vector encoding either c-Met (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BCI-3E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with c-Met transfected cells.)
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using BCI-3E7 Cat.# GM- 1005. BOSC23 cells were transiently transfected with an expression vector encoding either c-Met (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BCI-3E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with c-Met transfected cells.)
Testing Data #2
(Fig.2: SDS-PAGE analysis of purified BCI-3E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BCI-3C7 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Testing Data #2
(Fig.2: SDS-PAGE analysis of purified BCI-3E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BCI-3C7 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Galland F, Stefanova M, Lafage M, Birnbaum D (1992). Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23. Cytogenet. Cell Genet. 60 (2): 114–6.
Cooper CS (1992). The met oncogene: from detection by transfection to transmembrane receptor for hepatocyte growth factor. Oncogene 7 (1): 3–7.
NCBI and Uniprot Product Information
NCBI Description
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.
Protein type: Oncoprotein; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Met family
Chromosomal Location of Human Ortholog: 7q31
Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; basal plasma membrane
Molecular Function: protein binding; protein-tyrosine kinase activity; hepatocyte growth factor receptor activity; protein phosphatase binding; ATP binding
Biological Process: axon guidance; skeletal muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; myoblast proliferation; myotube differentiation; muscle cell migration; hepatocyte growth factor receptor signaling pathway; signal transduction; glucose homeostasis; liver development; cell proliferation; cell surface receptor linked signal transduction; positive chemotaxis; branching morphogenesis of a tube; adult behavior; positive regulation of transcription from RNA polymerase II promoter; brain development; endothelial cell morphogenesis; placenta development
Disease: Hepatocellular Carcinoma; Renal Cell Carcinoma, Papillary, 1
Research Articles on MET
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Product Notes
The MET met (Catalog #AAA468026) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-c Met monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Met can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA. Flow Cytometry: 1.2 ug/10^6 cells CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the MET met for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Met, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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