DKK1 recombinant protein

DKK1, 32-266aa, Human His tag, E Coli (Denatured)

Cat. Num. AAA205352

• Gene Names: DKK1; SK; DKK-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DKK1; 32-266aa; Human His tag; E Coli (Denatured); Dickkopf-related protein 1; DKK-1; SK; DKK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRH
• Sequence Length: 266

• Applicable Applications for DKK1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DKK1 recombinant protein

Dickkopf-related protein 1, also known as DKK1, antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Recombinant human DKK1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for DKK1 recombinant protein

Development

References

Ahn V.E., et al. (2011) Dev. Cell. 21:862-873; Haniu M., et al. (2011) Protein Sci. 20:1802-1813;

NCBI and Uniprot Product Information

• NCBI GI #: 7110719
• NCBI GeneID: 22943
• NCBI Accession #: NP_036374
• NCBI GenBank Nucleotide #: NM_012242.3
• UniProt Accession #: O94907; B2RC19
• Molecular Weight: 28.2 kDa (258aa)
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1
• UniProt Protein Name: Dickkopf-related protein 1
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; hDkk-1

NCBI Description

This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]

Uniprot Description

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity ().