Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-6C3 Cat.# GM- 0205. BOSC23 cells were transiently transfected with an expression vector encoding either Granzyme K (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-6C3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme K transfected cells.)
Mouse anti-Human Granzyme K Monoclonal Antibody | anti-GZMK antibody
anti-human Granzyme K monoclonal antibody
ELISA: 1:200 - 1:400
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-6C3 Cat.# GM- 0205. BOSC23 cells were transiently transfected with an expression vector encoding either Granzyme K (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-6C3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme K transfected cells.)
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-6C3 Cat.# GM- 0205. BOSC23 cells were transiently transfected with an expression vector encoding either Granzyme K (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-6C3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme K transfected cells.)
Testing Data #2
(Fig2: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an antimyc monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, GM-6C3 hybridoma supernatant was tested on all transfectants. A positive signal was obtained only with Granzyme K transfected cells (red curves).)
Testing Data #2
(Fig2: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an antimyc monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, GM-6C3 hybridoma supernatant was tested on all transfectants. A positive signal was obtained only with Granzyme K transfected cells (red curves).)
Bade B, Boettcher HE, Lohrmann J, Hink-Schauer C, Bratke K, Jenne DE, Virchow JC Jr and W. Luttmann (2005). Differential expression of the granzymes A, K and M and perforin in human peripheral blood lymphocytes. Int Immunol. (11): 1419-1428.
NCBI and Uniprot Product Information
NCBI Description
This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]
Uniprot Description
GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]
Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.-
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: extracellular region
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on GZMK
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Product Notes
The GZMK gzmk (Catalog #AAA468035) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Granzyme K monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Granzyme K can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA) pair with GM26E7 (catcher), Flow Cytometry (FC/FACS), CELISA, ELISA (EIA). Immunofluorescence: genetic immunisation with cDNA encoding human Granzyme K ELISA: 1:200 - 1:400 Flow Cytometry: 1.2 ug/10^6 cells CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the GZMK gzmk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granzyme K, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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