Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-5F4 Cat.#MBS5468025. MBS5468025 cells were transiently transfected with an expression vector encoding either FasL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-5F4 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with FasL transfected cells.)
Mouse anti-Human FASL Monoclonal Antibody | anti-FASLG antibody
anti-human FAS ligand (FasL) monoclonal antibody
ELISA: 1:200 - 1:400
For each application a titration should be determine the optimal concentration.
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-5F4 Cat.#MBS5468025. MBS5468025 cells were transiently transfected with an expression vector encoding either FasL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-5F4 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with FasL transfected cells.)
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-5F4 Cat.#MBS5468025. MBS5468025 cells were transiently transfected with an expression vector encoding either FasL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-5F4 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with FasL transfected cells.)
Testing Data #2
(SDS-PAGE analysis of GM-5F4 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-5F4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-5F4 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Testing Data #2
(SDS-PAGE analysis of GM-5F4 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-5F4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-5F4 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Suda T,Hashimoto H, Tanaka M, Ochi T, Nagata S (1997). Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing. J Exp Med 186(12):2045-50
Nagata S and Golstein P (1995). The Fas death factor. Science 10;267(5203):1449-56
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008]
Uniprot Description
FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Apoptosis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: extracellular space; lysosomal lumen; perinuclear region of cytoplasm; integral to plasma membrane; extracellular region; plasma membrane; caveola; nucleus; external side of plasma membrane
Molecular Function: protein binding; death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; signal transduction; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; cell-cell signaling; induction of apoptosis via death domain receptors; positive regulation of neuron apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; immune response; retinal cell programmed cell death
Disease: Lung Cancer; Autoimmune Lymphoproliferative Syndrome
Research Articles on FASLG
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Product Notes
The FASLG faslg (Catalog #AAA468025) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human FAS ligand (FasL) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FASL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC)and CELISA. Flow Cytometry: 1.2 ug/106 cells ELISA: 1:200 - 1:400 For each application a titration should be determine the optimal concentration. Researchers should empirically determine the suitability of the FASLG faslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FASL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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