Elastase 2A Recombinant Protein | ELA2A recombinant protein

Recombinant Elastase 2A (ELA2A)

Cat. Num. AAA2031272

• Gene Names: CELA2A; PE-1; ELA2A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Elastase 2A; Recombinant Elastase 2A (ELA2A); ELA2A recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; CGYP TYEVEDDVSR VVGGQEATPN TWPWQVSLQVLSSGRWRHNC GGSLVANNWV LTAAHCLSNY QTYRVLLGAH SLSNPGAGSAAVQVSKLVVH QRWNSQNVGN GYDIALIKLA SPVTLSKNIQ TACLPPAGTILPRNYVCYVT GWGLLQTNGN SPDTLRQGRL LVVDYATCSS ASWWGSSVKSSMVCAGGDGV TSSCNGDSGG PLNCRASNGQ WQVHGIVSFG SSLGCNYPRKPSVFTRVSNY IDWINSVMAR N
• Sequence Length: 269

• Applicable Applications for ELA2A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.0kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Cys17~Asn271
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119572112
• NCBI GeneID: 63036
• Molecular Weight: 28,888 Da
• NCBI Official Full Name: elastase 2A
• NCBI Official Synonym Full Names: chymotrypsin-like elastase family, member 2A
• NCBI Official Symbol: CELA2A
• NCBI Official Synonym Symbols: PE-1; ELA2A
• NCBI Protein Information: chymotrypsin-like elastase family member 2A
• UniProt Protein Name: Chymotrypsin-like elastase family member 2A
• UniProt Gene Name: CELA2A
• UniProt Synonym Gene Names: ELA2A
• UniProt Entry Name: CEL2A_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. [provided by RefSeq, May 2009]

Uniprot Description

CELA2A: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.71; Protease

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular region

Molecular Function: serine hydrolase activity; serine-type endopeptidase activity

Biological Process: proteolysis