SDS-Page
(AbFlex HA-Tag antibody (rAb) tested by Immunoprecipitation. Whole-cell lysate (400 ug) from U2OS cells expressing an HA-Tagged protein, was immunoprecipitated with 2 ug of AbFlex HA-Tag rAb (lane 1) or control IgG2a (lane 2). Immunoprecipitates were run on SDS-PAGE and analyzed by Western blot using HA-Tag mAb at 4 ug/ml.)
Mouse anti-Human HA Antibody | anti-HA antibody
AbFlex HA-Tag antibody (rAb)
Reactivity
Human
Applications
Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
HA; AbFlex HA-Tag antibody (rAb); anti-HA antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-HA antibody
Immunoprecipitation (IP)
Application Notes
Applications Validated:
IP: 2-5ug per IP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
IP: 2-5ug per IP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Tag
HA-Tag
Immunogen
This antibody was raised against a peptide containing multiple repeats of the HA tag epitope YPYDVPDYA.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex HA-Tag antibody (rAb) tested by Immunoprecipitation. Whole-cell lysate (400 ug) from U2OS cells expressing an HA-Tagged protein, was immunoprecipitated with 2 ug of AbFlex HA-Tag rAb (lane 1) or control IgG2a (lane 2). Immunoprecipitates were run on SDS-PAGE and analyzed by Western blot using HA-Tag mAb at 4 ug/ml.)
SDS-Page
(AbFlex HA-Tag antibody (rAb) tested by Immunoprecipitation. Whole-cell lysate (400 ug) from U2OS cells expressing an HA-Tagged protein, was immunoprecipitated with 2 ug of AbFlex HA-Tag rAb (lane 1) or control IgG2a (lane 2). Immunoprecipitates were run on SDS-PAGE and analyzed by Western blot using HA-Tag mAb at 4 ug/ml.)
Related Product Information for anti-HA antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex HA-Tag antibody (rAb) was raised in a Mouse host. It has been validated for use in Immunoprecipitation; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex HA-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HA-Tag has been derived from the hemagglutinin protein of the human influenza virus for use as an epitope for the detection and purification of tagged recombinant proteins expressed in vivo. The HA-Tag is a small epitope and generally does not interfere with the normal function of recombinant proteins.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex HA-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HA-Tag has been derived from the hemagglutinin protein of the human influenza virus for use as an epitope for the detection and purification of tagged recombinant proteins expressed in vivo. The HA-Tag is a small epitope and generally does not interfere with the normal function of recombinant proteins.
Product Categories/Family for anti-HA antibody
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The HA (Catalog #AAA389493) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex HA-Tag antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HA can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Applications Validated: IP: 2-5ug per IP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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