Western Blot (WB)
(ETO / RUNX1T1 antibody (pAb) tested by Immunoprecipitation. 10 ul of ETO / RUNX1T1 antibody was used to immunoprecipitate ETO / RUNX1T1 from 250 ug of K562 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ETO / RUNX1T1 antibody at a dilution of 1:500.)
Rabbit anti-Human ETO/RUNX1T1 Polyclonal Antibody | anti-ETO/RUNX1T1 antibody
ETO/RUNX1T1 antibody (pAb)
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
ETO/RUNX1T1; Polyclonal Antibody; ETO/RUNX1T1 antibody (pAb); CDR; MTG8; AML1T1; ZMYND2; CBFA2T1; anti-ETO/RUNX1T1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-ETO/RUNX1T1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 10ul per IP
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
IP: 10ul per IP
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This ETO/RUNX1T1 antibody was raised against a peptide within the internal region of human ETO/RUNX1T1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(ETO / RUNX1T1 antibody (pAb) tested by Immunoprecipitation. 10 ul of ETO / RUNX1T1 antibody was used to immunoprecipitate ETO / RUNX1T1 from 250 ug of K562 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ETO / RUNX1T1 antibody at a dilution of 1:500.)
Western Blot (WB)
(ETO / RUNX1T1 antibody (pAb) tested by Immunoprecipitation. 10 ul of ETO / RUNX1T1 antibody was used to immunoprecipitate ETO / RUNX1T1 from 250 ug of K562 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ETO / RUNX1T1 antibody at a dilution of 1:500.)
Western Blot (WB)
(ETO / RUNX1T1 antibody (pAb) tested by Western Blot: K-562 nuclear extract (20 ug per lane) probed with the ETO / RUNX1T1 antibody (pAb) at a dilution of 1:500.)
Western Blot (WB)
(ETO / RUNX1T1 antibody (pAb) tested by Western Blot: K-562 nuclear extract (20 ug per lane) probed with the ETO / RUNX1T1 antibody (pAb) at a dilution of 1:500.)
Related Product Information for anti-ETO/RUNX1T1 antibody
Short Description: Our ETO/RUNX1T1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: ETO/RUNX1T1 (AML1T1, CBFA2T1, MTG8, ZMYND2) belongs to a family of evolutionarily conserved nuclear factors that act as a transcriptional corepressor. ETO/RUNX1T1 does not interact directly with DNA but is recruited by transcription factors such as PLZF, BCL6, TAL1/SCL, Gfi1and Heb recruit nuclear co-repressors such as NCoR, SIN3, and SMRT/NCoR2, which in turn interact with histone deacetylase (HDAC) to mediate transcriptional repression. ETO/RUNX1T1 contains four evolutionarily conserved functional domains called nervy homology regions (NHRs) involved in protein-protein interaction. ETO/RUNX1T1 is frequently found as a fusion partner of AML1 in acute myeloid leukemia with the t(8;21) translocation which gives rise to the AML-ETO fusion protein.
Background: ETO/RUNX1T1 (AML1T1, CBFA2T1, MTG8, ZMYND2) belongs to a family of evolutionarily conserved nuclear factors that act as a transcriptional corepressor. ETO/RUNX1T1 does not interact directly with DNA but is recruited by transcription factors such as PLZF, BCL6, TAL1/SCL, Gfi1and Heb recruit nuclear co-repressors such as NCoR, SIN3, and SMRT/NCoR2, which in turn interact with histone deacetylase (HDAC) to mediate transcriptional repression. ETO/RUNX1T1 contains four evolutionarily conserved functional domains called nervy homology regions (NHRs) involved in protein-protein interaction. ETO/RUNX1T1 is frequently found as a fusion partner of AML1 in acute myeloid leukemia with the t(8;21) translocation which gives rise to the AML-ETO fusion protein.
Product Categories/Family for anti-ETO/RUNX1T1 antibody
NCBI and Uniprot Product Information
Molecular Weight
68kDa
Similar Products
Product Notes
The ETO/RUNX1T1 (Catalog #AAA388763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ETO/RUNX1T1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ETO/RUNX1T1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 10ul per IP WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the ETO/RUNX1T1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETO/RUNX1T1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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