Western Blot (WB)
(MGMT antibody (pAb) tested by Immunoprecipitation. 10 ul of MGMT antibody was used to immunoprecipitate MGMT from 250 ug of MCF-7 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MGMT antibody at a dilution of 1:500.)
Rabbit anti-Human MGMT Polyclonal Antibody | anti-MGMT antibody
MGMT antibody (pAb)
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
MGMT; Polyclonal Antibody; MGMT antibody (pAb); anti-MGMT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-MGMT antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 10ul per IP
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
IP: 10ul per IP
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This MGMT antibody was raised against a peptide derived from the C-terminal region of human MGMT.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(MGMT antibody (pAb) tested by Immunoprecipitation. 10 ul of MGMT antibody was used to immunoprecipitate MGMT from 250 ug of MCF-7 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MGMT antibody at a dilution of 1:500.)
Western Blot (WB)
(MGMT antibody (pAb) tested by Immunoprecipitation. 10 ul of MGMT antibody was used to immunoprecipitate MGMT from 250 ug of MCF-7 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MGMT antibody at a dilution of 1:500.)
Western Blot (WB)
(MGMT antibody (pAb) tested by Western blot. Nuclear extract of MCF-7 cells (20 ug) probed with MGMT antibody (pAb) at a dilution of 1:500.)
Western Blot (WB)
(MGMT antibody (pAb) tested by Western blot. Nuclear extract of MCF-7 cells (20 ug) probed with MGMT antibody (pAb) at a dilution of 1:500.)
Related Product Information for anti-MGMT antibody
Short Description: Our MGMT antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: MGMT (O-6 methylguanine DNA methyltransferase) is an enzyme involved in DNA damage repair. MGMT transfers methyl groups from O-6 methylguanine methylated DNA, and other methylated adducts, to a cysteine residue in itself, thus reversing DNA damage caused by alkylation. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT helps prevent DNA alkylation events from causing mutations. It also contributes to resistance to chemotherapy in cancer patients being treated with alkylating drugs.
Background: MGMT (O-6 methylguanine DNA methyltransferase) is an enzyme involved in DNA damage repair. MGMT transfers methyl groups from O-6 methylguanine methylated DNA, and other methylated adducts, to a cysteine residue in itself, thus reversing DNA damage caused by alkylation. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT helps prevent DNA alkylation events from causing mutations. It also contributes to resistance to chemotherapy in cancer patients being treated with alkylating drugs.
Product Categories/Family for anti-MGMT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
MGMT
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase
Protein Family
NCBI Description
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
Research Articles on MGMT
Similar Products
Product Notes
The MGMT (Catalog #AAA388645) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGMT antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGMT can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 10ul per IP WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the MGMT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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