Western Blot (WB)
(BCL11A / CTIP1 antibody (pAb) tested by Immunoprecipitation. 10 ul of BCL11A / CTIP1 antibody was used to immunoprecipitate BCL11A / CTIP1 from 250 ug of Raji nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the BCL11A / CTIP1 antibody at a dilution of 1:1,000.)
Rabbit anti-Human BCL11A/CTIP1 Polyclonal Antibody | anti-BCL11A/CTIP1 antibody
BCL11A/CTIP1 antibody (pAb)
IP: 5-10ul per IP
WB: 1:1,000-1:5,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Western Blot (WB)
(BCL11A / CTIP1 antibody (pAb) tested by Immunoprecipitation. 10 ul of BCL11A / CTIP1 antibody was used to immunoprecipitate BCL11A / CTIP1 from 250 ug of Raji nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the BCL11A / CTIP1 antibody at a dilution of 1:1,000.)
Western Blot (WB)
(BCL11A / CTIP1 antibody (pAb) tested by Immunoprecipitation. 10 ul of BCL11A / CTIP1 antibody was used to immunoprecipitate BCL11A / CTIP1 from 250 ug of Raji nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the BCL11A / CTIP1 antibody at a dilution of 1:1,000.)
Western Blot (WB)
(BCL11A / CTIP1 antibody (pAb) tested by Western blot. Nuclear extract of Raji cells (20 ug per lane) probed with BCL11A / CTIP1 antibody at a dilution of 1:1,000.)
Western Blot (WB)
(BCL11A / CTIP1 antibody (pAb) tested by Western blot. Nuclear extract of Raji cells (20 ug per lane) probed with BCL11A / CTIP1 antibody at a dilution of 1:1,000.)
Background: BCL11A/CTIP1 (B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1) is a transcriptional regulator, a myeloid and B-cell proto-oncogene and plays important roles in leukemogenesis and hematopoiesis. BCL11A/CTIP1 is involved in regulating hemoglobin gene expression. BCL11A regulates hemoglobin gene switching during fetal development and silencing of g-globin expression in adults. BCL11A interacts with the Mi-2/NuRD chromatin remodeling complexes, as well as the erythroid transcription factors GATA1 and FOG1. Abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Bcl-11A: Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 2p16.1
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein homodimerization activity; protein heterodimerization activity; metal ion binding; transcription corepressor activity
Biological Process: protein sumoylation; transcription, DNA-dependent; B cell differentiation; regulation of dendrite development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of collateral sprouting; negative regulation of transcription from RNA polymerase II promoter; negative regulation of axon extension; positive regulation of collateral sprouting; negative regulation of protein homooligomerization; T cell differentiation
Research Articles on BCL11A/CTIP1
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Product Notes
The BCL11A/CTIP1 bcl11a (Catalog #AAA388736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCL11A/CTIP1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCL11A/CTIP1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 5-10ul per IP WB: 1:1,000-1:5,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the BCL11A/CTIP1 bcl11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL11A/CTIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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