Western Blot (WB)
(ELP3 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (20 ug) probed with ELP3 antibody (pAb) at a dilution of 1:500.)
Rabbit anti-Human ELP3 Polyclonal Antibody | anti-ELP3 antibody
ELP3 antibody (pAb)
Gene Names
ELP3; KAT9
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
ELP3; Polyclonal Antibody; ELP3 antibody (pAb); KAT9; anti-ELP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
0.814ug/ul (varies by lot)
Applicable Applications for anti-ELP3 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This ELP3 antibody was raised against a peptide within the N-terminal half of human ELP3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(ELP3 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (20 ug) probed with ELP3 antibody (pAb) at a dilution of 1:500.)
Western Blot (WB)
(ELP3 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (20 ug) probed with ELP3 antibody (pAb) at a dilution of 1:500.)
Related Product Information for anti-ELP3 antibody
Short Description: Our ELP3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: ELP3 (Elongator Protein 3, also called KAT9) was originally identified as a component of the RNA polymerase II elongator complex, involved in transcriptional elongation by RNA Pol II. Elongator, through ELP3 may play a role in chromatin remodeling and is involved in acetylation of histones H3 and possibly H4. ELP3 may also play a role as a DNA demethylase in vertebrates, as it is important for paternal DNA demethylation.
Background: ELP3 (Elongator Protein 3, also called KAT9) was originally identified as a component of the RNA polymerase II elongator complex, involved in transcriptional elongation by RNA Pol II. Elongator, through ELP3 may play a role in chromatin remodeling and is involved in acetylation of histones H3 and possibly H4. ELP3 may also play a role as a DNA demethylase in vertebrates, as it is important for paternal DNA demethylation.
Product Categories/Family for anti-ELP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,202 Da
NCBI Official Full Name
elongator complex protein 3 isoform 1
NCBI Official Synonym Full Names
elongator acetyltransferase complex subunit 3
NCBI Official Symbol
ELP3
NCBI Official Synonym Symbols
KAT9
NCBI Protein Information
elongator complex protein 3; elongation protein 3 homolog
UniProt Protein Name
Elongator complex protein 3
Protein Family
UniProt Gene Name
ELP3
UniProt Synonym Gene Names
hELP3
UniProt Entry Name
ELP3_HUMAN
Uniprot Description
ELP3: a histone acetyltransferase. A subunit of the Elongator complex, which associates with the elongating, hyperphosphorylated form of RNA polymerase II.
Protein type: EC 2.3.1.48; Acetyltransferase
Chromosomal Location of Human Ortholog: 8p21.1
Cellular Component: transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; nucleolus; histone acetyltransferase complex
Molecular Function: protein binding; H4 histone acetyltransferase activity; metal ion binding; iron-sulfur cluster binding; phosphorylase kinase regulator activity; H3 histone acetyltransferase activity
Biological Process: tRNA wobble uridine modification; regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; central nervous system development; RNA elongation from RNA polymerase II promoter; neuron migration; regulation of protein kinase activity; positive regulation of cell migration
Similar Products
Product Notes
The ELP3 elp3 (Catalog #AAA388644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ELP3 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ELP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the ELP3 elp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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