Western Blot (WB)
(HOXA9 antibody (pAb) tested by Western blot. K-562 nuclear extract (20 ug per lane) probed with HOXA9 antibody at a dilution of 1:1,000.)
Rabbit anti-Human HOXA9 Polyclonal Antibody | anti-HOXA9 antibody
HOXA9 antibody (pAb)
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Western Blot (WB)
(HOXA9 antibody (pAb) tested by Western blot. K-562 nuclear extract (20 ug per lane) probed with HOXA9 antibody at a dilution of 1:1,000.)
Western Blot (WB)
(HOXA9 antibody (pAb) tested by Western blot. K-562 nuclear extract (20 ug per lane) probed with HOXA9 antibody at a dilution of 1:1,000.)
Background: HOXA9 (Homeobox protein A9, HOX1G) is a sequence-specific DNA binding transcription factor with a central role in both hematopoiesis and leukemia. HOXA9 is required for normal hematopoiesis and contributes to myeloid blood cell differentiation. High levels of HOXA9 expression in hematopoietic cells is a characteristic feature of acute myeloid leukemia (AML), and may contribute to the development of this disease. Overexpression of HOXA9 markedly expands hematopoietic stem cells.
NCBI and Uniprot Product Information
NCBI Description
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. Read-through transcription exists between this gene and the upstream homeobox A10 (HOXA10) gene.[provided by RefSeq, Mar 2011]
Uniprot Description
HOXA9: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98. A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2. Belongs to the Abd-B homeobox family.
Protein type: DNA-binding; Oncoprotein
Chromosomal Location of Human Ortholog: 7p15.2
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus
Molecular Function: protein binding; enzyme binding; sequence-specific DNA binding
Biological Process: embryonic forelimb morphogenesis; negative regulation of myeloid cell differentiation; transcription, DNA-dependent; multicellular organismal development; embryonic skeletal development; male gonad development; uterus development; endothelial cell activation; anterior/posterior pattern formation; regulation of transcription, DNA-dependent; mammary gland development; single fertilization; spermatogenesis; proximal/distal pattern formation
Research Articles on HOXA9
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Product Notes
The HOXA9 hoxa9 (Catalog #AAA388594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HOXA9 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOXA9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the HOXA9 hoxa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOXA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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