Rabbit anti-Human TIEG1/KLF10 Polyclonal Antibody | anti-TIEG1/KLF10 antibody

TIEG1/KLF10 antibody (pAb)

Cat. Num. AAA388767

• Gene Names: KLF10; EGRA; TIEG; TIEG1; EGR-alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: TIEG1/KLF10; Polyclonal Antibody; TIEG1/KLF10 antibody (pAb); EGRA; TIEG; EGR-alpha; anti-TIEG1/KLF10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-TIEG1/KLF10 antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1:500-1:1,000 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
• Immunogen: This TIEG1/KLF10 antibody was raised against a peptide derived from human TIEG1/KLF10.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(TIEG / KLF10 antibody (pAb) tested by Western blot K-562 nuclear extract (20 ug per lane) probed with the TIEG1 / KLF10 antibody (pAb) at a dilution of 1:500.)

Related Product Information for anti-TIEG1/KLF10 antibody

Short Description: Our TIEG1/KLF10 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.

Background: TIEG1/KLF10 (Transforming growth factor b-inducible early gene 1, Kruppel-like factor 10) is a transcriptional regulator involved in the control of cell growth. KLF10 binds to Sp1/GC rich DNA sequences and can activate or repress the transcription of a number of genes. KLF10 is especially important for osteoblast function, including bone mineralization and support of osteoclast differentiation. KLF10 is rapidly induced by TGFb, bone morphogenetic protein, estrogen and epidermal growth factor. KLF10 plays a major role in TGFb-mediated inhibition of cell proliferation and inflammation and induction of apoptosis.

Product Categories/Family for anti-TIEG1/KLF10 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5032177
• NCBI GeneID: 7071
• NCBI Accession #: NP_005646; AB_2615031
• NCBI GenBank Nucleotide #: NM_005655
• UniProt Accession #: Q13118
• Molecular Weight: 52kDa
• NCBI Official Full Name: Krueppel-like factor 10 isoform a
• NCBI Official Synonym Full Names: Kruppel like factor 10
• NCBI Official Symbol: KLF10
• NCBI Official Synonym Symbols: EGRA; TIEG; TIEG1; EGR-alpha
• NCBI Protein Information: Krueppel-like factor 10
• UniProt Protein Name: Krueppel-like factor 10
• UniProt Gene Name: KLF10
• UniProt Synonym Gene Names: TIEG; TIEG1; TGFB-inducible early growth response protein 1; TIEG-1
• UniProt Entry Name: KLF10_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]