Western Blot (WB)
(HMGA2 / HMGI-C antibody (pAb) tested by Immunoprecipitation. 10 ul of HMGA2 / HMGI-C antibody was used to immunoprecipitate HMGA2 / HMGI-C from 250 ug of HepG2 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the HMGA2 / HMGI-C antibody at a dilution of 1:3,000.)
Rabbit anti-Human, Mouse HMGA2/HMGI-C Polyclonal Antibody | anti-HMGA2/HMGI-C antibody
HMGA2/HMGI-C antibody (pAb)
IP: 10ul per IP
WB: 1:1,000-1:5,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Western Blot (WB)
(HMGA2 / HMGI-C antibody (pAb) tested by Immunoprecipitation. 10 ul of HMGA2 / HMGI-C antibody was used to immunoprecipitate HMGA2 / HMGI-C from 250 ug of HepG2 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the HMGA2 / HMGI-C antibody at a dilution of 1:3,000.)
Western Blot (WB)
(HMGA2 / HMGI-C antibody (pAb) tested by Immunoprecipitation. 10 ul of HMGA2 / HMGI-C antibody was used to immunoprecipitate HMGA2 / HMGI-C from 250 ug of HepG2 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the HMGA2 / HMGI-C antibody at a dilution of 1:3,000.)
Western Blot (WB)
(HMGA2 / HMGI-C antibody (pAb) tested by Western blot. Detection of HMGA2 / HMGI-C by Western blot. Nuclear extract (25 ug) of mouse ES cells probed with HMGI-C / HMGA2 antibody at a dilution of 1:3,000.)
Western Blot (WB)
(HMGA2 / HMGI-C antibody (pAb) tested by Western blot. Detection of HMGA2 / HMGI-C by Western blot. Nuclear extract (25 ug) of mouse ES cells probed with HMGI-C / HMGA2 antibody at a dilution of 1:3,000.)
Background: HMGA2 (High-mobility group AT-hook protein 2, HMGI-C) is a member of the HMG (High mobility group) protein family, which are non-histone chromatin-associated proteins involved in DNA organization and regulation of transcription. The HMG protein family consists of six proteins and is subdivided into three subfamilies: the HMG-1/-2 subfamily, the HMG-I/Y subfamily and the HMG-14/-17 subfamily. Mouse models of HMGA2 function indicate that it is involved in proliferation at the cellular level and obesity at the organismal level. Overexpression of HMGA2 is observed in a variety of cancers.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
HMGA2: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Interacts with E4F1. Interacts with NEK2. Belongs to the HMGA family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Oncoprotein
Chromosomal Location of Human Ortholog: 12q15
Cellular Component: nucleoplasm; nuclear chromosome; nucleus
Molecular Function: protein binding; nucleosomal DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; AT DNA binding; DNA-dependent protein kinase activity; 5'-deoxyribose-5-phosphate lyase activity; SMAD binding; DNA bending activity; transcription factor binding
Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; mesodermal cell differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; base-excision repair; regulation of growth; chondrocyte differentiation; negative regulation of retroviral genome replication; mitosis; transcription, DNA-dependent; heterochromatin formation; response to virus; DNA damage response, detection of DNA damage; stem cell differentiation; DNA catabolic process, endonucleolytic; negative regulation of DNA binding; chromosome condensation; mesenchymal cell differentiation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; chromosome breakage; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Disease: Leiomyoma, Uterine
Research Articles on HMGA2/HMGI-C
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Product Notes
The HMGA2/HMGI-C hmga2 (Catalog #AAA388731) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGA2/HMGI-C antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HMGA2/HMGI-C can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 10ul per IP WB: 1:1,000-1:5,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the HMGA2/HMGI-C hmga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGA2/HMGI-C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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