SDS-Page
(Recombinant HDAC6 protein gel. HDAC6 protein was run on a 7.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 133.8 kDa Purity: > 90%)
HDAC6 recombinant protein
Recombinant HDAC6 protein
Synonyms
HDAC6; Recombinant HDAC6 protein; HDAC-6; Histone Deacetylase 6; HD6; HDAC6 recombinant protein
Host
Baculovirus
Form/Format
Recombinant HDAC6 protein is supplied at a concentration of 0.2ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant HDAC6 protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant HDAC6 protein gel. HDAC6 protein was run on a 7.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 133.8 kDa Purity: > 90%)
SDS-Page
(Recombinant HDAC6 protein gel. HDAC6 protein was run on a 7.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 133.8 kDa Purity: > 90%)
Testing Data
(HTRF assay for Recombinant HDAC6 activity 1 uM H3K9ac (1-21 aa) peptide was incubated with different concentrations of HDAC6 protein in a 10 ul reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 30 min at 37 degree , then 10 &miccro;l H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for Recombinant HDAC6 activity 1 uM H3K9ac (1-21 aa) peptide was incubated with different concentrations of HDAC6 protein in a 10 ul reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 30 min at 37 degree , then 10 &miccro;l H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for Recombinant HDAC6 activity 1 ?M H3K9ac (1-21 aa) peptide was incubated with different concentrations of HDAC6 protein in a 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 30 min at 37 ?, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for Recombinant HDAC6 activity 1 ?M H3K9ac (1-21 aa) peptide was incubated with different concentrations of HDAC6 protein in a 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 30 min at 37 ?, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection.)
Related Product Information for HDAC6 recombinant protein
Short Description: Recombinant human HDAC6 was expressed in a baculovirus expression system as the full-length protein (accession number NP_001308154.1) with a C-terminal DYKDDDDK-Tag. The molecular weight of the protein is 135.7 kDa. The recombinant protein is >85% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: HDAC6 (Histone Deacetylase 6) is a member of the class IIb mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones or many cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue.In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).HDAC6 is a unique enzyme that harbors a full duplication of its deacetylase homology region, which appears to contribute independently to the overall activity of HDAC6 protein. Alpha-tubulin and HSP90 are two known substrates of HDAC6, playing an important role in cellular mechanisms related to the microtubule network and HSP90-dependent events. HDAC6 also participates in regulating expression of a group of genes involved in the remodeling of chromatin during cell differentiation.
Background: HDAC6 (Histone Deacetylase 6) is a member of the class IIb mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones or many cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue.In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).HDAC6 is a unique enzyme that harbors a full duplication of its deacetylase homology region, which appears to contribute independently to the overall activity of HDAC6 protein. Alpha-tubulin and HSP90 are two known substrates of HDAC6, playing an important role in cellular mechanisms related to the microtubule network and HSP90-dependent events. HDAC6 also participates in regulating expression of a group of genes involved in the remodeling of chromatin during cell differentiation.
Product Categories/Family for HDAC6 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 133.8kDa
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Product Notes
The HDAC6 (Catalog #AAA388247) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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